Ryang Yong Suk, Yang Eun Ju, Kim Jeong-Lye, Lee Kyu-Jae, Sung Ho-Joong, Kim Jong-Bae, Kim In Sik
Department of Biomedical Laboratory Science, Institute of Health Science, College of Health Science, Yonsei University, Wonju, 220-701, Republic of Korea.
Parasitol Res. 2007 Sep;101(4):1103-10. doi: 10.1007/s00436-007-0591-y. Epub 2007 Jul 6.
Although Echinostoma hortense is one of the intestinal trematodes with a high infection rate in South Korea, the exact immune response against E. hortense infection has yet to be fully investigated. In the present study, we investigated differential susceptibilities in two different strains of micenamely, BALB/c (H-2d) and C3H/HeN (H-2k) mice. Likewise, we investigated the effects of ketotifen, an antiallergic drug, on the immune response against E. hortense infection. The worm recovery rate of the C3H/HeN mice was much higher than that of the BALB/c mice. The messenger ribonucleic acid (mRNA) expressions of interleukin (IL)-4 and IL-5 in the BALB/c mice were stronger than that of the C3H/HeN mice after E. hortense infection, but IL-1beta and tumor necrosis factor (TNF)-alpha expressions in the BALB/c mice were weaker than that of the C3H/HeN mice after E. hortense infection. The number of goblet cells and eosinophils increased after E. hortense infection in the BALB/c and the C3H/HeN mice. The worm recovery rate was higher and lasted longer in the ketotifen-treated mice in comparison to the untreated mice. Ketotifen suppressed the mRNA expression of IL-4 and IL-5 in the BALB/c mice, but did not in the C3H/HeN mice. The IL-1beta expressions were inhibited by ketotifen in the two strains, but TNF-alpha expression was inhibited in the C3H/HeN mice after ketotifen treatment. In addition, ketotifen inhibited the increase in eosinophils and goblet cells in varying degrees, depending on the strain. In summary, the immune sensitivity against E. hortense depends on the species of the host. The ketotifen treatment administered on the infected mice differently blocked the immune response against E. hortense infection.
虽然棘口吸虫是韩国感染率较高的肠道吸虫之一，但针对棘口吸虫感染的确切免疫反应尚未得到充分研究。在本研究中，我们调查了两种不同品系小鼠——即BALB/c（H-2d）和C3H/HeN（H-2k）小鼠的易感性差异。同样，我们研究了抗过敏药物酮替芬对针对棘口吸虫感染的免疫反应的影响。C3H/HeN小鼠的虫体回收率远高于BALB/c小鼠。棘口吸虫感染后，BALB/c小鼠中白细胞介素（IL）-4和IL-5的信使核糖核酸（mRNA）表达强于C3H/HeN小鼠，但棘口吸虫感染后，BALB/c小鼠中IL-1β和肿瘤坏死因子（TNF）-α的表达弱于C3H/HeN小鼠。棘口吸虫感染后，BALB/c和C3H/HeN小鼠中的杯状细胞和嗜酸性粒细胞数量增加。与未治疗的小鼠相比，酮替芬治疗的小鼠虫体回收率更高且持续时间更长。酮替芬抑制了BALB/c小鼠中IL-4和IL-5的mRNA表达，但对C3H/HeN小鼠没有抑制作用。两种品系中，酮替芬均抑制了IL-1β的表达，但酮替芬治疗后，C3H/HeN小鼠中的TNF-α表达受到抑制。此外，酮替芬根据品系不同程度地抑制了嗜酸性粒细胞和杯状细胞的增加。总之，对棘口吸虫的免疫敏感性取决于宿主的种类。对感染小鼠施用酮替芬对针对棘口吸虫感染的免疫反应有不同程度的阻断作用。
