Hu Kun, Cui Fuzhai, Lv Qiang, Ma Jun, Feng Qingling, Xu Li, Fan Daidi
Department of Materials Science and Engineering, Tsinghua University, Beijing 100084, People's Republic of China.
J Biomed Mater Res A. 2008 Feb;84(2):483-90. doi: 10.1002/jbm.a.31440.
Recombinant human-like collagen (RHLC) was added to fibroin solution to prepare a novel hybrid scaffold material for skin tissue engineering. The morphology of the scaffold had highly homogeneous and interconnected pores with pore sizes 136 +/- 32 mum measured by scanning electron microscopy (SEM). FTIR analysis indicated intermolecular crosslinkages between fibroin and RHLC formed. The viscosity of the blend solution increased because of the interaction between fibroin and RHLC, and then it restrained the unwanted fibroin aggregation in freezing process that generally appeared in fibroin scaffold preparation with freeze drying method. After methanol treatment the fibroin/RHLC scaffold became water-stable. The porosity of scaffolds was >>90%, the compressive strength and modulus were up to 662 +/- 32 KPa and 7.8 +/- 0.64 MPa, respectively. Fibroblasts cultured within fibroin/RHLC scaffolds were investigated by SEM, laser scanning confocal microscopy (LSCM), and MTT assay, which showed that the adding of RHLC significantly enhanced the cells adhesion, proliferation, and viability compare with fibroin scaffolds. These results suggest that the hybrid scaffolds have favorable characteristics for skin tissue engineering.
将重组类人胶原蛋白（RHLC）添加到丝素蛋白溶液中，以制备用于皮肤组织工程的新型混合支架材料。通过扫描电子显微镜（SEM）测量，该支架的形态具有高度均匀且相互连通的孔隙，孔径为136±32μm。傅里叶变换红外光谱（FTIR）分析表明丝素蛋白与RHLC之间形成了分子间交联。由于丝素蛋白与RHLC之间的相互作用，混合溶液的粘度增加，进而抑制了冷冻过程中通常在采用冷冻干燥法制备丝素蛋白支架时出现的丝素蛋白不必要的聚集。经过甲醇处理后，丝素蛋白/RHLC支架变得水稳定。支架的孔隙率>>90%，抗压强度和模量分别高达662±32 KPa和7.8±0.64 MPa。通过SEM、激光扫描共聚焦显微镜（LSCM）和MTT法对在丝素蛋白/RHLC支架内培养的成纤维细胞进行了研究，结果表明与丝素蛋白支架相比，添加RHLC显著增强了细胞的粘附、增殖和活力。这些结果表明，该混合支架具有用于皮肤组织工程的良好特性。
