Lu Xiong, Wang Yingbo, Yang Xiudong, Zhang Qiyi, Zhao Zhanfeng, Weng Lu-Tao, Leng Yang
Key Lab of Advanced Technologies of Materials, Ministry of Education, School of Materials Science and Engineering, Southwest Jiaotong University, Chengdu 610031, China.
J Biomed Mater Res A. 2008 Feb;84(2):523-34. doi: 10.1002/jbm.a.31471.
In the present study, surface functional groups of titanium surfaces gone through different treatments, including acid etched treatment (AE), nitric acid treatment (NT), heat treatment (HT), and alkali treatment (AT), and their behaviors in vitro and in vivo was thoroughly studied by spectroscopic analysis. In vitro and in vivo results revealed that the rank of bioactivity of various surfaces was AE < NT < HT < AT. XPS analysis indicated that AT greatly increased the OH group concentration on the titanium surface whereas HT reduced the OH group concentration. Thus, OH group difference could not be a good explanation of bioactivity difference. On the other hand, ToF-SIMS analysis demonstrated the TiOH+/Ti+ ratios of various surfaces correlated well with the bioactivity and the surface energies, which implied that Ti-OH could play an important role in the bioactivity. This detail investigation of the relationship between surface functional groups and surface bioactivity could help us to broaden the knowledge about the mechanism of bioactivity and to design next generation bioactive materials.
在本研究中，对经过不同处理的钛表面的表面官能团进行了研究，这些处理包括酸蚀处理（AE）、硝酸处理（NT）、热处理（HT）和碱处理（AT），并通过光谱分析对其在体外和体内的行为进行了深入研究。体外和体内结果表明，各种表面的生物活性排序为AE < NT < HT < AT。X射线光电子能谱（XPS）分析表明，碱处理极大地增加了钛表面的羟基浓度，而热处理降低了羟基浓度。因此，羟基差异并不能很好地解释生物活性差异。另一方面，飞行时间二次离子质谱（ToF-SIMS）分析表明，各种表面的TiOH+/Ti+比率与生物活性和表面能密切相关，这意味着Ti-OH可能在生物活性中起重要作用。对表面官能团与表面生物活性之间关系的详细研究有助于我们拓宽对生物活性机制的认识，并设计下一代生物活性材料。
