Vrana Nihal E, Elsheikh Ahmed, Builles Nicolas, Damour Odile, Hasirci Vasif
METU, BIOMAT, Department of Biological Sciences, Biotechnology Research Unit, 06531 Ankara, Turkey.
Biomaterials. 2007 Oct;28(29):4303-10. doi: 10.1016/j.biomaterials.2007.06.013. Epub 2007 Jul 6.
Collagen-based micropatterned films were seeded with human corneal keratocyte and epithelial cells to study their mechanical properties as tissue engineering substrates. The patterns were in the form of parallel channels with slanted walls. Influence of cell presence, type and growth on the mechanical properties of the films was investigated. Unseeded films showed gradual strength reduction from an initial value of 0.046 N/mm, possibly due to degradation, down to 0.032+/-0.012 N/mm in 2 weeks. Keratocyte growth was found to significantly improve the mechanical behavior of the films upon 1 week of incubation (0.067+/-0.017 N/mm) and the improvement continued gradually over the next 2 weeks. Films seeded with D407 retinal pigment epithelial cells, on the other hand, experienced a decrease (0.023+/-0.011 N/mm), followed by a slight increase in mechanical properties in the 21-day period. A steady increase in the number of keratocytes along the channels, cytoskeleton alignment and extracellular matrix (ECM) secretion restricted to the channels was observed. Increase in strength observed with keratocytes and, to a lesser extent, with the epithelial cells can be attributed to directional ECM synthesis and the orientation of the cells and their cytoskeleton which contribute to the strength in the direction of the channels. This study showed that cell, especially keratocyte, presence compensates for the degradation of collagen films and improve the overall mechanical properties of the engineered tissue.
基于胶原蛋白的微图案薄膜接种了人角膜基质细胞和上皮细胞，以研究其作为组织工程基质的力学性能。这些图案为具有倾斜壁的平行通道形式。研究了细胞的存在、类型和生长对薄膜力学性能的影响。未接种细胞的薄膜强度从初始值0.046 N/mm逐渐降低，可能是由于降解，在2周内降至0.032±0.012 N/mm。发现角膜基质细胞生长在培养1周后显著改善了薄膜的力学行为（0.067±0.017 N/mm），并且在接下来的2周内逐渐持续改善。另一方面，接种D407视网膜色素上皮细胞的薄膜在21天内经历了强度降低（0.023±0.011 N/mm），随后力学性能略有增加。观察到沿通道的角膜基质细胞数量稳步增加、细胞骨架排列以及细胞外基质（ECM）分泌局限于通道。角膜基质细胞以及在较小程度上上皮细胞导致的强度增加可归因于定向的ECM合成以及细胞及其细胞骨架的取向，这有助于在通道方向上增强强度。这项研究表明，细胞，尤其是角膜基质细胞的存在弥补了胶原薄膜的降解并改善了工程组织的整体力学性能。
