Jing Gu, Yan Zhenguang, Li Yu, Xie Liping, Zhang Rongqing
Institute of Marine Biotechnology, Department of Biological Sciences and Biotechnology, Tsinghua University, Beijing, China.
Mar Biotechnol (NY). 2007 Sep-Oct;9(5):650-9. doi: 10.1007/s10126-007-9018-0. Epub 2007 Jul 7.
Distribution of an acid phosphatase, AcPase I, from pearl oyster (Pinctada fucata) in different tissues was investigated via enzyme activity determination and immunohistochemistry. Positive reactions were observed in sections of digestive gland, base of gill filaments, and epithelia of the outer side of the middle fold and the inner side of the outer fold, which indicated AcPase I might participate in processes besides immune defense, such as calcium metabolism or shell formation. Its effects on CaCO(3) crystal formation were studied in vitro. Results revealed that AcPase I inhibited CaCO(3) precipitation in a dose-dependent manner and had no affinity for calcium. CaCO(3) crystals induced by AcPase I exhibited a cluster needle-like morphology, which proved to be aragonite. The morphology and size of the aragonites varied with different concentrations of AcPase I. Our observations described here may provide important clues to further understanding of the correlations between mineralization and immune defense in the oyster.
通过酶活性测定和免疫组织化学方法，研究了珍珠贝（Pinctada fucata）中一种酸性磷酸酶AcPase I在不同组织中的分布情况。在消化腺切片、鳃丝基部以及中褶外侧和外褶内侧上皮中观察到阳性反应，这表明AcPase I可能除了参与免疫防御外，还参与钙代谢或贝壳形成等过程。在体外研究了其对CaCO₃晶体形成的影响。结果显示，AcPase I以剂量依赖方式抑制CaCO₃沉淀，且对钙无亲和力。AcPase I诱导形成的CaCO₃晶体呈现簇状针状形态，经证实为文石。文石的形态和大小随AcPase I浓度不同而变化。我们在此描述的观察结果可能为进一步理解牡蛎矿化与免疫防御之间的相关性提供重要线索。
