Lupini Caterina, Legnardi Matteo, Graziosi Giulia, Cecchinato Mattia, Listorti Valeria, Terregino Calogero, Catelli Elena
Department of Veterinary Medical Sciences, University of Bologna, 40064 Ozzano dell'Emilia, Italy.
Department of Animal Medicine, Production and Health, University of Padova, 35020 Legnaro, Italy.
Vaccines (Basel). 2023 Mar 22;11(3):708. doi: 10.3390/vaccines11030708.
Newcastle disease virus (NDV) and avian metapneumovirus (aMPV) are among the most impactful pathogens affecting the turkey industry. Since turkeys are routinely immunized against both diseases, the hatchery administration of the combined respective live vaccines would offer remarkable practical advantages. However, the compatibility of NDV and aMPV vaccines has not yet been experimentally demonstrated in this species. To address this issue, an aMPV subtype B live vaccine was administered to day-old poults either alone or in combination with one of two different ND vaccines. The birds were then challenged with a virulent aMPV subtype B strain, clinical signs were recorded and aMPV and NDV vaccine replication and humoral immune response were assessed. All results supported the absence of any interference hampering protection against aMPV, with no significant differences in terms of clinical scoring. In addition, the mean aMPV vaccine viral titers and antibody titers measured in the dual vaccinated groups were comparable or even higher than in the group vaccinated solely against aMPV. Lastly, based on the NDV viral and antibody titers, the combined aMPV and NDV vaccination does not seem to interfere with protection against NDV, although further studies involving an actual ND challenge will be necessary to fully demonstrate this hypothesis.
新城疫病毒（NDV）和禽偏肺病毒（aMPV）是影响火鸡养殖业的最具影响力的病原体之一。由于火鸡通常会针对这两种疾病进行免疫接种，因此在孵化场同时接种各自的联合活疫苗将具有显著的实际优势。然而，NDV和aMPV疫苗在该物种中的兼容性尚未通过实验得到证实。为了解决这个问题，将一种aMPV B亚型活疫苗单独或与两种不同的ND疫苗之一联合接种给一日龄雏火鸡。然后用强毒aMPV B亚型毒株对这些鸟类进行攻毒，记录临床症状，并评估aMPV和NDV疫苗的复制情况以及体液免疫反应。所有结果均表明不存在任何妨碍针对aMPV的保护作用的干扰，在临床评分方面没有显著差异。此外，在联合接种组中测得的平均aMPV疫苗病毒滴度和抗体滴度与仅接种aMPV疫苗的组相当，甚至更高。最后，基于NDV病毒和抗体滴度，aMPV和NDV联合接种似乎不会干扰针对NDV的保护作用，尽管需要进一步进行涉及实际ND攻毒的研究来充分证实这一假设。
