在无特定病原体雏鸡中同时应用新城疫活疫苗、传染性支气管炎疫苗和禽偏肺病毒疫苗后的免疫反应及相互作用。

Immune responses and interactions following simultaneous application of live Newcastle disease, infectious bronchitis and avian metapneumovirus vaccines in specific-pathogen-free chicks.

作者信息

Awad Faez, Forrester Anne, Baylis Matthew, Lemiere Stephane, Jones Richard, Ganapathy Kannan

机构信息

Institute of Infection and Global Health, University of Liverpool, Leahurst Campus, Neston, Cheshire CH64 7TE, UK; University of Omar Al-Mukhtar, Faculty of Veterinary Medicine, Al-Bayda, Libya.

Institute of Infection and Global Health, University of Liverpool, Leahurst Campus, Neston, Cheshire CH64 7TE, UK.

出版信息

Res Vet Sci. 2015 Feb;98:127-33. doi: 10.1016/j.rvsc.2014.11.004. Epub 2014 Nov 18.

DOI:10.1016/j.rvsc.2014.11.004

PMID:25482324

Abstract

Interactions between live Newcastle disease virus (NDV), avian metapneumovirus (aMPV) and infectious bronchitis virus (IBV) vaccines following simultaneous vaccination of day old specific pathogen free (SPF) chicks were evaluated. The chicks were divided into eight groups: seven vaccinated against NDV, aMPV and IBV (single, dual or triple) and one unvaccinated as control. Haemagglutination inhibition (HI) NDV antibody titres were similar across all groups but were above protective titres. aMPV vaccine when given with other live vaccines suppressed levels of aMPV enzyme-linked immunosorbent assay (ELISA) antibodies. Cellular and local immunity induced by administration of NDV, aMPV or IBV vaccines (individually or together) showed significant increase in CD4+, CD8+ and IgA bearing B-cells in the trachea compared to the unvaccinated group. Differences between the vaccinated groups were insignificant. Simultaneous vaccination with live NDV, aMPV and IBV did not affect the protection conferred against aMPV or IBV.

摘要

对一日龄无特定病原体（SPF）雏鸡同时接种新城疫病毒（NDV）、禽偏肺病毒（aMPV）和传染性支气管炎病毒（IBV）疫苗后的相互作用进行了评估。雏鸡被分为八组：七组接种针对NDV、aMPV和IBV的疫苗（单苗、二联苗或三联苗），一组未接种作为对照。所有组的血凝抑制（HI）新城疫病毒抗体滴度相似，但均高于保护滴度。aMPV疫苗与其他活疫苗同时接种时，会抑制aMPV酶联免疫吸附测定（ELISA）抗体水平。与未接种组相比，单独或联合接种NDV、aMPV或IBV疫苗诱导的细胞免疫和局部免疫显示，气管中CD4⁺、CD8⁺和表达IgA的B细胞显著增加。接种组之间的差异不显著。同时接种活的NDV、aMPV和IBV疫苗不影响对aMPV或IBV的保护效果。