Tan Thean Yen, Poh Karen, Ng Siew Yong
Department of Microbiology, Laboratory Medicine Services, Changi General Hospital, Singapore.
Infect Control Hosp Epidemiol. 2007 Aug;28(8):941-4. doi: 10.1086/518964. Epub 2007 Jun 19.
To investigate the molecular epidemiology of carbapenem-resistant Acinetobacter baumannii-calcoaceticus complex isolates in a tertiary care hospital where the prevalence of carbapenem resistance among these organisms is high.
The study was a prospective, observational study performed during an 8-month period (May 1 through December 31, 2004). A. baumannii isolates recovered from all clinical samples during the study period were included in the study. Antibiotic susceptibility testing was performed using the disk diffusion method, and all carbapenem-resistant strains were typed by a polymerase chain reaction-based typing method.Setting. An 800-bed hospital in Singapore.
More than half of recovered isolates were clonally unrelated, with the remaining isolates grouped into 4 genotypes.
The results of the study suggest that the high prevalence of carbapenem resistance among Acinetobacter organisms in this institution is not caused by the spread of a predominant clone and that other factors may need to be investigated.
在一家三级护理医院中，耐碳青霉烯类鲍曼不动杆菌-醋酸钙不动杆菌复合体分离株的分子流行病学情况，该医院中这些微生物的耐碳青霉烯类发生率较高。
本研究为前瞻性观察性研究，于8个月期间（2004年5月1日至12月31日）进行。研究纳入了研究期间从所有临床样本中分离出的鲍曼不动杆菌。采用纸片扩散法进行药敏试验，所有耐碳青霉烯类菌株通过基于聚合酶链反应的分型方法进行分型。地点：新加坡一家拥有800张床位的医院。
超过一半的分离株在克隆上不相关，其余分离株分为4种基因型。
研究结果表明，该机构中不动杆菌属微生物耐碳青霉烯类的高发生率并非由优势克隆的传播所致，可能需要研究其他因素。
