拟南芥中SOS3与SOS2调节结构域的复合物：克隆、表达、纯化、结晶及初步X射线分析。
The complex between SOS3 and SOS2 regulatory domain from Arabidopsis thaliana: cloning, expression, purification, crystallization and preliminary X-ray analysis.
作者信息
Sánchez-Barrena María José, Moreno-Pérez Sandra, Angulo Iván, Martínez-Ripoll Martín, Albert Armando
机构信息
Grupo de Cristalografía Macromolecular y Biología Estructural, Instituto de Química Física Rocasolano, Consejo Superior de Investigaciones Científicas, Serrano 119, E-28006 Madrid, Spain.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2007 Jul 1;63(Pt 7):568-70. doi: 10.1107/S174430910702533X. Epub 2007 Jun 11.
Abstract
The salt-tolerance genes SOS3 (salt overly sensitive 3) and SOS2 (salt overly sensitive 2) regulatory domain of Arabidopsis thaliana were cloned into a polycistronic plasmid and the protein complex was expressed in Escherichia coli, allowing purification to homogeneity in three chromatographic steps. Crystals were grown using vapour-diffusion techniques. The crystals belonged to space group P2(1)2(1)2(1), with unit-cell parameters a = 44.14, b = 57.39, c = 141.90 A.
摘要
将拟南芥的耐盐基因SOS3（盐过度敏感3）和SOS2（盐过度敏感2）调控域克隆到一个多顺反子质粒中，并在大肠杆菌中表达该蛋白质复合物，通过三步色谱法可将其纯化至同质状态。使用气相扩散技术培养晶体。这些晶体属于空间群P2(1)2(1)2(1)，晶胞参数为a = 44.14、b = 57.39、c = 141.90 Å。
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