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J Mol Biol. 2011 Nov 18;414(1):135-44. doi: 10.1016/j.jmb.2011.09.041. Epub 2011 Oct 1.

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1
Early abscisic acid signal transduction mechanisms: newly discovered components and newly emerging questions.早期脱落酸信号转导机制：新发现的组分和新出现的问题。
Genes Dev. 2010 Aug 15;24(16):1695-708. doi: 10.1101/gad.1953910.
2
Abscisic acid: emergence of a core signaling network.脱落酸：核心信号网络的出现。
Annu Rev Plant Biol. 2010;61:651-79. doi: 10.1146/annurev-arplant-042809-112122.
3
XDS.XDS.（这个词如果没有更多背景信息，很难准确翻译出更有意义的内容，直接保留原文是一种处理方式，或者音译为“克斯达斯”之类，但感觉都不太符合常规翻译场景，你可以补充更多关于这个词的信息以便我更准确翻译 ）
Acta Crystallogr D Biol Crystallogr. 2010 Feb;66(Pt 2):125-32. doi: 10.1107/S0907444909047337. Epub 2010 Jan 22.
4
Molecular replacement with MOLREP.使用MOLREP进行分子置换。
Acta Crystallogr D Biol Crystallogr. 2010 Jan;66(Pt 1):22-5. doi: 10.1107/S0907444909042589. Epub 2009 Dec 21.
5
A protein kinase-phosphatase pair interacts with an ion channel to regulate ABA signaling in plant guard cells.蛋白激酶-磷酸酶对与离子通道相互作用，以调节植物保卫细胞中的 ABA 信号转导。
Proc Natl Acad Sci U S A. 2009 Dec 15;106(50):21419-24. doi: 10.1073/pnas.0910601106. Epub 2009 Dec 2.
6
Structural mechanism of abscisic acid binding and signaling by dimeric PYR1.二聚体 PYR1 结合和信号转导的脱落酸结构机制。
Science. 2009 Dec 4;326(5958):1373-9. doi: 10.1126/science.1181829. Epub 2009 Oct 22.
7
In vitro reconstitution of an abscisic acid signalling pathway.脱落酸信号通路的体外重建
Nature. 2009 Dec 3;462(7273):660-4. doi: 10.1038/nature08599. Epub 2009 Nov 18.
8
Structural basis of abscisic acid signalling.脱落酸信号传导的结构基础。
Nature. 2009 Dec 3;462(7273):609-14. doi: 10.1038/nature08583.
9
Protein phosphatases 2C regulate the activation of the Snf1-related kinase OST1 by abscisic acid in Arabidopsis.蛋白磷酸酶 2C 通过脱落酸调节拟南芥中 Snf1 相关激酶 OST1 的激活。
Plant Cell. 2009 Oct;21(10):3170-84. doi: 10.1105/tpc.109.069179. Epub 2009 Oct 23.
10
Threonine at position 306 of the KAT1 potassium channel is essential for channel activity and is a target site for ABA-activated SnRK2/OST1/SnRK2.6 protein kinase.丝氨酸 306 位是 KAT1 钾通道的活性必需位点，也是 ABA 激活的 SnRK2/OST1/SnRK2.6 蛋白激酶的靶位。
Biochem J. 2009 Dec 10;424(3):439-48. doi: 10.1042/BJ20091221.

拟南芥SnRK2.6/OST1：K50N和D160A突变体的克隆、表达、纯化、结晶及初步X射线分析

SnRK2.6/OST1 from Arabidopsis thaliana: cloning, expression, purification, crystallization and preliminary X-ray analysis of K50N and D160A mutants.

作者信息

Yunta Cristina, Martinez-Ripoll Martin, Albert Armando

机构信息

Departamento de Cristalografía y Biología Estructural, Instituto de Química Física Rocasolano, Consejo Superior de Investigaciones Científicas, Serrano 119, 28006 Madrid, Spain.

出版信息

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Mar 1;67(Pt 3):364-8. doi: 10.1107/S1744309110053807. Epub 2011 Feb 25.

DOI:10.1107/S1744309110053807
PMID:21393844
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3053164/
Abstract

The SnRK2.6 (SNF1-related kinase 2.6) gene from Arabidopsis thaliana encodes the serine/threonine protein kinase SnRK2.6/OST1 (OPEN STOMATA 1). It plays a central role in the drought-tolerance mechanism. OST1 is in fact the main positive effector in the hydric stress response. The SnRK2.6 gene was cloned into the pGEX4T1 plasmid, mutated and expressed in Escherichia coli, allowing purification to homogeneity in two chromatographic steps. Various OST1 mutants yielded crystals using vapour-diffusion techniques, but only one mutant showed a good diffraction pattern. Its crystals diffracted to 2.8 Å resolution and belonged to space group P222(1), with unit-cell parameters a=77.7, b=99.4, c=108.4 Å. A promising molecular-replacement solution was found using the structure of the kinase domain of the yeast AMP-activated protein kinase SNF1 (PDB entry 3hyh) as the search model.

摘要

来自拟南芥的SnRK2.6（蔗糖非发酵-1相关蛋白激酶2.6）基因编码丝氨酸/苏氨酸蛋白激酶SnRK2.6/OST1（开放气孔1）。它在耐旱机制中起核心作用。事实上，OST1是水分胁迫响应中的主要正向效应因子。SnRK2.6基因被克隆到pGEX4T1质粒中，进行突变并在大肠杆菌中表达，通过两步色谱法可将其纯化至同质状态。使用气相扩散技术，各种OST1突变体都产生了晶体，但只有一个突变体显示出良好的衍射图谱。其晶体衍射分辨率达到2.8 Å，属于空间群P222(1)，晶胞参数a=77.7、b=99.4、c=108.4 Å。以酵母AMP激活的蛋白激酶SNF1的激酶结构域结构（PDB登录号3hyh）作为搜索模型，找到了一个有前景的分子置换解决方案。