Verlinde P H C J, Oey I, Hendrickx M E, Van Loey A M, Temme E H M
Centre for Food and Microbial Technology, Laboratory of Food Technology, Department of Microbial and Molecular Systems, Katholieke Universiteit Leuven, Kasteelpark Arenberg 22, Heverlee, Belgium.
Eur J Clin Nutr. 2008 Oct;62(10):1224-30. doi: 10.1038/sj.ejcn.1602840. Epub 2007 Jul 11.
To investigate the effect of simultaneous administration of [6S]-5-methyltetrahydrofolic acid ([6S]-5-CH(3)H(4)PteGlu) with L-ascorbic acid (L-AA) on serum folate concentrations in healthy male subjects.
A total of nine healthy male volunteers were recruited. Serum folate concentrations were measured before and up to 8 h after administration of each treatment (1) placebo, (2) 343 microg [6S]-5-CH(3)H(4)PteGlu), (3) 343 microg [6S]-5-CH(3)H(4)PteGlu) with 289.4 mg L-AA and (4) 343 microg [6S]-5-CH(3)H(4)PteGlu) with 973.8 mg L-AA (n=10 samples per treatment).
Serum folate concentrations significantly increased compared with baseline values, starting from 30 min after [6S]-5-CH(3)H(4)PteGlu administration and remained significantly higher than baseline values during the first 6 h for treatments 3 and 4, and during the first 4 h for treatment 2. Maximal serum folate responses were observed between 0.5 and 1.5 h after [6S]-5-CH(3)H(4)PteGlu consumption and significantly differed between treatments 2 and 4 (P<0.05). When [6S]-5-CH(3)H(4)PteGlu was concurrently administered with 289.4 or 973.8 mg L-AA, the total serum folate response, calculated as the area under the curve (AUC), was significantly improved (46.5+/-4.0 and 53.0+/-4.0 vs 34.3+/-3.8 h nmol/l, P<0.05). No significant difference in AUC was found between the 289.4 and the 973.8 mg L-AA treatments.
Administration of a physiological dose of [6S]-5-CH(3)H(4)PteGlu with L-AA significantly improved the measured serum folate response in folate saturated healthy men.
研究同时给予[6S]-5-甲基四氢叶酸（[6S]-5-CH(3)H(4)PteGlu）和L-抗坏血酸（L-AA）对健康男性受试者血清叶酸浓度的影响。
共招募了9名健康男性志愿者。在给予每种治疗（1）安慰剂、（2）343微克[6S]-5-CH(3)H(4)PteGlu、（3）343微克[6S]-5-CH(3)H(4)PteGlu加289.4毫克L-AA以及（4）343微克[6S]-5-CH(3)H(4)PteGlu加973.8毫克L-AA）之前及之后长达8小时测量血清叶酸浓度（每种治疗n = 10个样本）。
与基线值相比，血清叶酸浓度显著升高，从给予[6S]-5-CH(3)H(4)PteGlu后30分钟开始，对于治疗3和4，在最初6小时内以及对于治疗2在最初4小时内均显著高于基线值。在摄入[6S]-5-CH(3)H(4)PteGlu后0.5至1.5小时观察到最大血清叶酸反应，且治疗2和4之间存在显著差异（P<0.05）。当[6S]-5-CH(3)H(4)PteGlu与289.4或973.8毫克L-AA同时给药时，以曲线下面积（AUC）计算的总血清叶酸反应显著改善（46.5±4.0和53.0±4.0对比34.3±3.8小时纳摩尔/升，P<0.05）。在289.4毫克和973.8毫克L-AA治疗之间未发现AUC有显著差异。
给予生理剂量的[6S]-5-CH(3)H(4)PteGlu和L-AA可显著改善叶酸饱和的健康男性中测得的血清叶酸反应。
