de Ambrosis A, Vishnumohan S, Paterson J, Haber P, Arcot J
Food Science and Technology, School of Chemical Engineering, UNSW Australia, Sydney, New South Wales, Australia.
Drug Health Services, Sydney South West Area Health Service, Discipline of Addiction Medicine, Faculty of Medicine, Royal Prince Alfred Hospital, Sydney, New South Wales, Australia.
Eur J Clin Nutr. 2017 Jan;71(1):103-106. doi: 10.1038/ejcn.2016.122. Epub 2016 Jul 27.
BACKGROUND/OBJECTIVES: The aim of the study was to measure the relative bioavailability of labeled pteroylglutamic acid (13C5-PteGlu) from a pectin-coated fortified rice in vivo to measure any effect of the edible coating on folic acid bioavailability.
SUBJECTS/METHODS: Healthy volunteers (N=26) aged 18-39 years received three test meals in three randomized short-term cross-over trials: Trial 1: aqueous 400 μg 13C5-PteGlu, Trial 2: 200 g cooked white rice+400 μg 13C5-PteGlu,Trial 3: 200 g fortified cooked white rice with pectin-coated premix containing 400 μg 13C5-PteGlu. Blood samples were drawn at 0,1,2,5 and 8 h postprandial. The concentration of 13C5-5 methyl-tetrahydrofolate appearing in plasma was quantified using high performance liquid chromatography-mass spectrometry (MS)/MS. For 24 h before baseline estimation and during the area under the curve (AUC) study, the subjects were placed on a low folate diet (∼100 μg/day). The relative bioavailability of the folic acid following Trial 3 was measured by comparing the 13C5-5 methyl-tetrahydrofuran (THF) AUC with Trials 1 and 2.
The bioavailability of folic acid in a pectin-coated rice premix was 68.7% (range 47-105) and 86.5% (range 65-115) in uncoated fortified rice relative to aqueous folic acid.
This study is the first demonstration of the bioavailability of folate in pectin-coated fortified rice in humans.
背景/目的：本研究旨在测定体内来自果胶包衣强化米的标记蝶酰谷氨酸（13C5 - 蝶酰谷氨酸）的相对生物利用度，以评估可食用包衣对叶酸生物利用度的影响。
受试者/方法：18 - 39岁的健康志愿者（N = 26）在三项随机短期交叉试验中接受了三顿测试餐：试验1：400μg 13C5 - 蝶酰谷氨酸水溶液；试验2：200g煮熟的白米饭 + 400μg 13C5 - 蝶酰谷氨酸；试验3：200g含有400μg 13C5 - 蝶酰谷氨酸的果胶包衣预混料强化煮熟白米饭。餐后0、1、2、5和8小时采集血样。使用高效液相色谱 - 质谱联用仪（MS）/ MS对血浆中出现的13C5 - 5 - 甲基四氢叶酸浓度进行定量。在基线估计前24小时和曲线下面积（AUC）研究期间，受试者采用低叶酸饮食（约100μg/天）。通过将试验3后叶酸的13C5 - 5 - 甲基四氢呋喃（THF）AUC与试验1和试验2进行比较，测量叶酸的相对生物利用度。
相对于叶酸水溶液，果胶包衣大米预混料中叶酸的生物利用度为68.7%（范围47 - 105），未包衣强化米中为86.5%（范围65 - 115）。
本研究首次证明了人类食用果胶包衣强化米中叶酸的生物利用度。
