Sartini Davide, Santarelli Andrea, Rossi Valentina, Goteri Gaia, Rubini Corrado, Ciavarella Domenico, Lo Muzio Lorenzo, Emanuelli Monica
Institute of Biochemical Biotechnologies, Polytechnic University of Marche, Ancona, Italy.
Mol Med. 2007 Jul-Aug;13(7-8):415-21. doi: 10.2119/2007–00035.Sartini.
We investigated expression levels of Nicotinamide N-Methyltransferase (NNMT), an enzyme involved in the biotransformation of many drugs and xenobiotic compounds, in oral squamous cell carcinoma (OSCC). Measurements were performed by semi-quantitative RT-PCR and quantitative real-time PCR in tumor and matched adjacent healthy tissue. Interestingly, NNMT was up-regulated in most of the favorable OSCCs, while no marked NNMT expression alterations between tumor and normal mucosa were detected in most of the unfavorable OSCCs. Western blot and immunohistochemical analyses also were performed and the relationship between tumor characteristics and NNMT levels in OSCC were studied to evaluate the effectiveness of NNMT as a prognostic marker in the squamous cell carcinoma of the oral cavity. In summary, the present study suggests that NNMT may have potential as a biomarker and a therapeutic target for OSCC.
我们研究了烟酰胺N-甲基转移酶（NNMT）在口腔鳞状细胞癌（OSCC）中的表达水平，该酶参与多种药物和外源性化合物的生物转化。通过半定量逆转录聚合酶链反应（RT-PCR）和定量实时PCR对肿瘤组织及其配对的相邻健康组织进行检测。有趣的是，在大多数预后良好的OSCC中，NNMT表达上调，而在大多数预后不良的OSCC中，未检测到肿瘤组织与正常黏膜之间NNMT表达有明显改变。我们还进行了蛋白质免疫印迹和免疫组织化学分析，并研究了OSCC中肿瘤特征与NNMT水平之间的关系，以评估NNMT作为口腔鳞状细胞癌预后标志物的有效性。总之，本研究表明NNMT可能具有作为OSCC生物标志物和治疗靶点的潜力。
