Cui Yanhong, Guo Zhouyi, Zhao Yanping, Zheng Ying, Qiao Yanfang, Cai Jiye, Liu Songhao
Photonic Chinese Medicine Laboratory, MOE Key Laboratory of Laser Life Science, South China Normal University, Guangzhou 510631, China.
Acta Biochim Biophys Sin (Shanghai). 2007 Jul;39(7):484-9. doi: 10.1111/j.1745-7270.2007.00309.x.
To find out the mechanism of modulating the deformability of erythrocytes with low intensity He-Ne laser action, we studied the effect of low intensity He-Ne laser on the ultrastructure of human erythrocyte membrane. Erythrocytes were treated with free radicals from a Fenton reaction system before exposing them to low intensity He-Ne laser. The ultrastructure of damaged erythrocyte membrane was examined by atomic force microscopy. The results showed that the erythrocyte membrane became very rough and the molecules on the surface of the membrane congregated into particles of different magnitudes sizes after treating with free radicals. Comparing the degree of congregation of the molecular particles in the non-irradiated group and the He-Ne laser irradiated (9 mW and 18 mW) group, we found the average size of molecular particles in the laser irradiated group was smaller than that in the non-irradiated group, indicating that the low intensity laser had repairing function to the damage of erythrocyte membrane produced by the free radicals.
为了探究低强度氦氖激光作用调节红细胞变形能力的机制，我们研究了低强度氦氖激光对人红细胞膜超微结构的影响。在将红细胞暴露于低强度氦氖激光之前，先用芬顿反应系统产生的自由基处理红细胞。用原子力显微镜检查受损红细胞膜的超微结构。结果表明，用自由基处理后，红细胞膜变得非常粗糙，膜表面的分子聚集成不同大小的颗粒。比较未照射组和氦氖激光照射（9毫瓦和18毫瓦）组分子颗粒的聚集程度，我们发现激光照射组分子颗粒的平均尺寸小于未照射组，这表明低强度激光对自由基产生的红细胞膜损伤具有修复作用。
