Departamento de Ciências Fisiológicas, Universidade Federal do Estado do Rio de Janeiro, Rio de Janeiro, Brazil.
Lasers Med Sci. 2012 Jan;27(1):211-7. doi: 10.1007/s10103-011-0945-7. Epub 2011 Jun 24.
Low-intensity laser therapy is based on the excitation of endogenous chromophores in biotissues and free-radical generation could be involved in its biological effects. In this work, the effects of the low-intensity infrared laser on plasma protein content and oxidative stress in blood from Wistar rats were studied. Blood samples from Wistar rats were exposed to low-intensity infrared laser in continuous wave and pulsed-emission modes at different fluencies. Plasma protein content and two oxidative stress markers (thiobarbituric acid-reactive species formation and myeloperoxidase activity) were carried out to assess the effects of laser irradiation on blood samples. Low-intensity infrared laser exposure increases plasma protein content, induces lipid peroxidation, and increases myeloperoxidase activity in a dose- and frequency-dependent way in blood samples. The low-intensity infrared laser increases plasma protein content and oxidative stress in blood samples, suggesting that laser therapy protocols should take into account fluencies, frequencies, and wavelengths of the laser before beginning treatment.
低强度激光疗法基于生物组织内内源性色团的激发，而自由基的产生可能与其生物学效应有关。在这项工作中，研究了低强度红外激光对 Wistar 大鼠血液中血浆蛋白含量和氧化应激的影响。将 Wistar 大鼠的血液样本分别暴露于连续波和脉冲发射模式的低强度红外激光下，采用不同的通量。通过测定两种氧化应激标志物（硫代巴比妥酸反应性物质形成和髓过氧化物酶活性）来评估激光照射对血液样本的影响。结果表明，低强度红外激光照射可增加血浆蛋白含量，诱导脂质过氧化，并以剂量和频率依赖的方式增加血液样本中的髓过氧化物酶活性。低强度红外激光增加了血液样本中的血浆蛋白含量和氧化应激，这表明在开始治疗之前，激光治疗方案应考虑激光的通量、频率和波长。
