Simpson Laura J, Sale Julian E
M.R.C. Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, UK.
Subcell Biochem. 2006;40:399-403. doi: 10.1007/978-1-4020-4896-8_34.
This method results in the differential staining of sister chromatids during replication and permits the direct visualisation of genetic exchanges between sister chromatids. It therefore provides a direct visual readout for crossover recombination. The generation of SCE is dependent on homologous recombination and is elevated in a number of circumstances including following exposure to DNA damaging agents and in some genetic backgrounds that result in increased dependence on recombination based pathways. It is important to remember that SCEs are probably only generated by a fraction of recombination events and their elevation may reflect increased use of recombination-based pathways or an increase in the resolution of recombination intermediates with cross-over.
这种方法在复制过程中导致姐妹染色单体的差异染色，并允许直接观察姐妹染色单体之间的基因交换。因此，它为交叉重组提供了直接的视觉读数。姐妹染色单体交换的产生依赖于同源重组，并且在多种情况下会升高，包括暴露于DNA损伤剂之后以及在一些导致对基于重组的途径依赖性增加的遗传背景中。重要的是要记住，姐妹染色单体交换可能仅由一部分重组事件产生，其升高可能反映了基于重组的途径使用增加或重组中间体交叉分辨率的增加。
