Rowland Owen, Lee Robert, Franke Rochus, Schreiber Lukas, Kunst Ljerka
Department of Botany, University of British Columbia, Vancouver, BC, Canada.
FEBS Lett. 2007 Jul 24;581(18):3538-44. doi: 10.1016/j.febslet.2007.06.065. Epub 2007 Jul 3.
The cuticle coats the aerial organs of land plants and is composed of a cutin matrix embedded and overlayed with waxes. The Arabidopsis CER3 gene is important for cuticular wax biosynthesis and was reported to correspond to At5g02310 encoding an E3 ubiquitin ligase. Here, we demonstrate that CER3 is not At5g02310 and instead corresponds to WAX2/YRE/FLP1 (At5g57800), a gene of unknown function required for wax biosynthesis. CER3 protein has also been implicated in cutin production because strong cer3 alleles display organ fusions. Leaf cutin analysis of two cer3 alleles did not reveal significant differences in cutin load or composition, indicating that CER3 has no major role in leaf cutin formation.
角质层覆盖着陆地植物的地上器官，由嵌入蜡质并覆盖蜡质的角质基质组成。拟南芥CER3基因对表皮蜡质生物合成很重要，据报道它对应于编码E3泛素连接酶的At5g02310。在这里，我们证明CER3不是At5g02310，而是对应于WAX2/YRE/FLP1（At5g57800），这是蜡质生物合成所需的一个功能未知的基因。CER3蛋白也与角质生成有关，因为强cer3等位基因表现出器官融合。对两个cer3等位基因的叶片角质分析未发现角质含量或组成有显著差异，表明CER3在叶片角质形成中没有主要作用。
