Du Kanghua, Li Yirong, Wang Lingmin, Zhang Da, Ma Jixian, Bao Lingfeng, Tang Zhengfu, Zhang Jie, Mu Wanfu, Yang Long
Institute of Tropical Eco-agriculture Science, Yunnan Academy of Agricultural Sciences, Yuanmou, Yunnan, 651300, China.
Yiliang County Agricultural Science and Technology Extension Service Centre, Kunming, Yunnan, 652100, China.
Sci Data. 2025 Aug 29;12(1):1511. doi: 10.1038/s41597-025-05816-w.
The leaf wax layer in cauliflower (Brassica oleracea var. Botrytis L.) and other Brassica crops play an important role in environmental adaptation and defense. Here, high-throughput RNA sequencing was performed on leaves of a wax-deficient mutant type (WL) and its wild type (YL). A total of 43.13 Gb of raw RNA-seq data was obtained, of which 42.38 Gb of high-quality clean reads were retained after quality control. A total of 24,529 genes and 1,092 long non-coding RNA (lncRNA) were identified through transcriptome assembly and annotation. Functional enrichment analysis indicated that these mRNA and lncRNA are associated with biotic stress responses, lipid biosynthesis, and fatty acid degradation pathways. This study provides valuable transcriptomic resources for the wax deficiency of cauliflower and lays a foundation for future research on genetic improvement and breeding strategies.
菜花（甘蓝变种. 花椰菜L.）和其他十字花科作物中的叶蜡层在环境适应和防御中发挥着重要作用。在此，对蜡质缺陷突变体类型（WL）及其野生型（YL）的叶片进行了高通量RNA测序。共获得43.13 Gb的原始RNA-seq数据，经过质量控制后保留了42.38 Gb的高质量 clean reads。通过转录组组装和注释共鉴定出24,529个基因和1,092个长链非编码RNA（lncRNA）。功能富集分析表明，这些mRNA和lncRNA与生物胁迫反应、脂质生物合成和脂肪酸降解途径相关。本研究为菜花蜡质缺陷提供了有价值的转录组资源，为未来的遗传改良和育种策略研究奠定了基础。
