Kumar K, Goodrich J, Marcoux F
Department of Pathology, Michigan State University, East Lansing 48824.
J Neurosci Methods. 1991 Aug;39(1):1-8. doi: 10.1016/0165-0270(91)90087-g.
Reduced blood supply in the hippocampus has been considered to be a factor contributory to this region's selective susceptibility to ischemia. To determine if there are differences in vascularization of vulnerable and resistant regions of the brain, a quantitative morphometric assessment of vascular filling was performed in the gerbil brain by using a relatively new and highly sensitive automated laser cytometric device, ACAS (Adherent Cell Analysis and Sorting). Evan's blue, an intravital vascular tracer, was injected intracardiac in 5 Mongolian gerbils to identify perfused blood vessels in the cortex and CA1 region of hippocampus. Exactly 3 min later, the animal was decapitated, the brain removed and sections prepared for fluorescent quantitative morphometric analysis of blood vessels. The perfused vessels appeared as fluorescent structures due to the autofluorescent property of Evan's blue. Several fields in each of the two specified regions were studied and integrated numbers of blood vessels/mm2 obtained for statistical comparisons. Control brains perfused with normal saline (vehicle for Evan's blue) were used to determine the background fluorescence. The number of perfused blood vessels of all sizes was found to be lower in the CA1 sector of hippocampus than that in the cerebral cortex. The number of capillary size vessels was also lower in the CA1 region but the difference was not significant. Since it is the capillaries that are of prime importance in the diffusion system, and we found no significant regional difference in the density of capillary size vessels, it appears unlikely that the peculiarities of patterns of vascularization contribute significantly to vulnerability to ischemia.
海马体血液供应减少被认为是该区域对缺血具有选择性易感性的一个促成因素。为了确定大脑易损区域和抗性区域的血管形成是否存在差异，我们使用一种相对较新且高度灵敏的自动激光细胞仪ACAS（粘附细胞分析和分选仪）对沙鼠大脑中的血管充盈进行了定量形态学评估。向5只蒙古沙鼠心内注射活体血管示踪剂伊文思蓝，以识别皮质和海马体CA1区域的灌注血管。正好3分钟后，将动物断头，取出大脑并制备切片，用于对血管进行荧光定量形态学分析。由于伊文思蓝的自发荧光特性，灌注血管呈现为荧光结构。对两个指定区域中的每个区域的几个视野进行了研究，并获得了每平方毫米血管的积分数量以进行统计比较。用灌注生理盐水（伊文思蓝的载体）的对照大脑来确定背景荧光。发现海马体CA1区所有大小的灌注血管数量均低于大脑皮质。CA1区毛细血管大小的血管数量也较低，但差异不显著。由于毛细血管在扩散系统中最为重要，并且我们发现毛细血管大小的血管密度在区域上没有显著差异，因此血管形成模式的特殊性似乎不太可能对缺血易感性有显著影响。
