Yang Xiupei, Yuan Hongyan, Wang Chunling, Su Xiaodong, Hu Li, Xiao Dan
College of Chemistry and College of Chemical Engineering, Sichuan University, Chengdu 610065, PR China.
J Pharm Biomed Anal. 2007 Oct 18;45(2):362-6. doi: 10.1016/j.jpba.2007.05.017. Epub 2007 May 25.
In this paper, a capillary electrophoresis (CE) system with in-column fiber optics light-emitting diode (LED) induced fluorescence detection was developed for the determination of penicillamine (PA). The influence of buffer concentration, buffer pH, applied voltage and injection time was systematically investigated. Optimum separation conditions were obtained with 10 mM borate buffer at pH 9.1, applied voltage 20 kV and 8 s hydrodynamic injection at 30 mbar. The detection system displayed linear dynamic range from 3.2 x 10(-7) to 4.8 x 10(-5) mol L(-1) with a correlation coefficient of 0.9991 and good repeatability (R.S.D.=2.46%). The method was applied to the determination of PA in commercial tablets and human plasma, which the recoveries of standard PA added to tablets and human plasma sample were found to be in the range of 96.26-102.68 and 91.10-99.35%, respectively. The proposed method is cheap, rapid, easy, and accurate, and can be successfully applied to the formulation analysis and bioanalysis.
本文开发了一种带有柱内光纤发光二极管（LED）诱导荧光检测的毛细管电泳（CE）系统，用于测定青霉胺（PA）。系统研究了缓冲液浓度、缓冲液pH值、施加电压和进样时间的影响。在pH 9.1的10 mM硼酸盐缓冲液、20 kV的施加电压和30 mbar下8 s的流体动力学进样条件下，获得了最佳分离条件。该检测系统的线性动态范围为3.2×10⁻⁷至4.8×10⁻⁵ mol L⁻¹，相关系数为0.9991，且具有良好的重复性（相对标准偏差=2.46%）。该方法应用于市售片剂和人血浆中PA的测定，发现添加到片剂和人血浆样品中的标准PA的回收率分别在96.26 - 102.68%和91.10 - 99.35%范围内。所提出的方法廉价、快速、简便且准确，可成功应用于制剂分析和生物分析。
