Gill D M
J Infect Dis. 1976 Mar;133 Suppl:55-63. doi: 10.1093/infdis/133.supplement_1.s55.
Peptide A1 of Vibrio cholerae toxin, nicotinamide adenine dinucleotide, adenosine triphosphate, and a soluble protein present in erythrocyte supernatant are required for the activation of pigeon erythrocyte ghost adenylate cyclase but are not required to maintain the activated state. The compounds are all required simultaneously, and when all are added to ghosts, adenylate cyclase activity increases at a linear rate without delay. Under optimal conditions significant activation of cyclase is given by less than one molecule of toxin per ghost. Intact cholera toxin may be inactive in vitro. There is a delay of about 1 min between the addition of intact toxin and the attainment of the final rate of increase of adenylate cyclase activity. During this period, glutathione reduces the disulfide bond between peptides A1 and A2. The delay is eliminated if the toxin is reduced before addition. More A1 is liberated if the toxin is also denatured with sodium dodecyl sulfate.
霍乱弧菌毒素的肽A1、烟酰胺腺嘌呤二核苷酸、三磷酸腺苷以及存在于红细胞上清液中的一种可溶性蛋白质是激活鸽红细胞空壳腺苷酸环化酶所必需的，但维持激活状态则不需要这些物质。这些化合物必须同时存在，当将它们全部添加到空壳中时，腺苷酸环化酶活性会立即以线性速率增加。在最佳条件下，每个空壳中少于一个毒素分子就能使环化酶得到显著激活。完整的霍乱毒素在体外可能无活性。在添加完整毒素与腺苷酸环化酶活性达到最终增加速率之间存在约1分钟的延迟。在此期间，谷胱甘肽会还原肽A1和A2之间的二硫键。如果在添加毒素之前将其还原，则延迟会消除。如果毒素还用十二烷基硫酸钠变性，则会释放出更多的A1。
