烟酰胺腺嘌呤二核苷酸在霍乱毒素体外作用中的参与情况。
Involvement of nicotinamide adenine dinucleotide in the action of cholera toxin in vitro.
作者信息
Gill D M
出版信息
Proc Natl Acad Sci U S A. 1975 Jun;72(6):2064-8. doi: 10.1073/pnas.72.6.2064.
Abstract
NAD is a necessary cofactor for the activation of adenylate cyclase (ATP pyrophosphate-lyase (cyclizing), EC 4.6.1.1) by cholera toxin. Lysates of certain types of cell that hydrolyze their endogenous store of NAD after cell disruption respond poorly or not at all to cholera toxin. Lysates of pigeon erythrocytes, which lack enzymes that degrade NAD, provide a convenient and reproducible system for assaying the activity of cholera toxin in vitro and allow investigation of the mechanism of action of the toxin upon broken cells.
摘要
NAD是霍乱毒素激活腺苷酸环化酶（ATP焦磷酸裂解酶（环化），EC 4.6.1.1）所必需的辅助因子。某些类型的细胞在破裂后会水解其内源性NAD储备，其裂解物对霍乱毒素反应不佳或完全无反应。鸽红细胞裂解物缺乏降解NAD的酶，为体外测定霍乱毒素活性提供了一个方便且可重复的系统，并有助于研究该毒素对破碎细胞的作用机制。
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