Schaller G Eric, Binder Brad M
Department of Biological Sciences, Dartmouth College, Hanover, New Hampshire, USA.
Methods Enzymol. 2007;422:270-87. doi: 10.1016/S0076-6879(06)22013-5.
The ethylene receptors of plants are related to and originated from bacterial histidine kinases. As such they represent a system by which one can study not only how the ethylene signal is perceived and its signal transduced, but also how bacterial two-component systems have been adapted for signal transduction in a eukaryote. Much of the biochemical characterization of the ethylene receptors, including the demonstration of kinase activity, ethylene binding, and interaction with other signaling components, has relied on the ability of the receptors to be functionally expressed in transgenic yeast. This chapter describes some of the key approaches used for such work, with a special emphasis on techniques employed to analyze ethylene binding. In many cases the approaches used in transgenic yeast may also be used for studies of the receptors in the native plant.
植物的乙烯受体与细菌组氨酸激酶相关且起源于细菌组氨酸激酶。因此，它们代表了一个系统，通过这个系统不仅可以研究乙烯信号是如何被感知以及其信号如何转导，还可以研究细菌双组分系统是如何适应在真核生物中进行信号转导的。乙烯受体的许多生化特性，包括激酶活性、乙烯结合以及与其他信号成分的相互作用的证明，都依赖于受体在转基因酵母中功能性表达的能力。本章描述了用于此类工作的一些关键方法，特别强调了用于分析乙烯结合的技术。在许多情况下，转基因酵母中使用的方法也可用于研究天然植物中的受体。
