Paasonen Lauri, Laaksonen Timo, Johans Christoffer, Yliperttula Marjo, Kontturi Kyösti, Urtti Arto
Department of Pharmaceutics, University of Kuopio, Kuopio, Finland.
J Control Release. 2007 Sep 11;122(1):86-93. doi: 10.1016/j.jconrel.2007.06.009. Epub 2007 Jun 21.
A novel proof of principle demonstration for contents release from liposomes that can be selectively activated by light irradiation is presented. The content release temperature was adjusted to slightly above body temperature, and hydrophobic or hydrophilic gold nanoparticles were incorporated into the lipid bilayer or the core of the liposomes, respectively. The release of a fluorescent marker was monitored upon exposure of the liposomes to UV light. Gold nanoparticle-containing liposomes remained intact at 37 degrees C but contents release was triggered by UV light-induced heating of the gold nanoparticles. This light-induced release is mediated by heat transfer from the gold nanoparticles to the lipids and subsequent phase transition. Heating is highly localized in the liposomes and the gold nanoparticles act as energy collectors that sensitize the liposomes to the light signal. This kind of selectivity is very advantageous as it can potentially make the drug delivery mechanism biologically more compatible. The triggered contents release could also be extended to other applications where local contents release is needed.
本文展示了一种新型的原理验证，即通过光照射可选择性激活的脂质体内容物释放。将内容物释放温度调节至略高于体温，疏水性或亲水性金纳米颗粒分别被掺入脂质体的脂质双层或核心中。在脂质体暴露于紫外光时监测荧光标记物的释放。含金纳米颗粒的脂质体在37摄氏度时保持完整，但紫外光诱导的金纳米颗粒加热触发了内容物释放。这种光诱导释放是由金纳米颗粒向脂质的热传递以及随后的相变介导的。加热高度局限于脂质体中，金纳米颗粒充当能量收集器，使脂质体对光信号敏感。这种选择性非常有利，因为它有可能使药物递送机制在生物学上更具兼容性。触发的内容物释放还可扩展到其他需要局部内容物释放的应用中。
