Wang Xianyan, Hu Xiao, Daley Andrea, Rabotyagova Olena, Cebe Peggy, Kaplan David L
Department of Biomedical Engineering, Tufts University, Medford, Massachusetts 02155, USA.
J Control Release. 2007 Aug 28;121(3):190-9. doi: 10.1016/j.jconrel.2007.06.006. Epub 2007 Jun 14.
An all-aqueous, stepwise deposition process with silk fibroin protein for the assembly of nanoscale layered controlled release coatings was exploited. Model compounds, Rhodamine B, Even Blue and Azoalbumin, representing small molecule drugs and therapeutically relevant proteins were incorporated in the nanocoating process and their loading and release behavior was quantified. In addition, the structure and morphology of the coatings were characterized. Release studies in vitro showed that control of beta-sheet crystal content and the multilayer structure of the silk coatings correlated with the release properties of the incorporated compounds. In particular, higher crystallinity and a thicker silk capping layer suppressed the initial burst of release and prolonged the duration of release. These novel coatings and deposition approach provide a unique option to regulate structure and morphology, and thus release kinetics. The results also suggest these systems as a promising framework for surface engineering of biomaterials and medical devices to regulate the release of drugs, when considered with the all-aqueous process involved, the conformal nature of the coatings, the robust material properties of silk fibroin, and the degradability and biocompatibility of this family of protein.
利用一种采用丝素蛋白的全水性逐步沉积工艺来组装纳米级分层控释涂层。在纳米涂层制备过程中加入了代表小分子药物和治疗相关蛋白质的模型化合物罗丹明B、伊文思蓝和偶氮白蛋白，并对它们的负载和释放行为进行了量化。此外，还对涂层的结构和形态进行了表征。体外释放研究表明，β-折叠晶体含量和丝素涂层的多层结构的控制与所掺入化合物的释放特性相关。特别是，更高的结晶度和更厚的丝素封端层抑制了初始释放突释，并延长了释放持续时间。这些新型涂层和沉积方法为调节结构和形态以及释放动力学提供了独特的选择。当考虑到所涉及的全水性工艺、涂层的保形性、丝素蛋白强大的材料特性以及该蛋白质家族的可降解性和生物相容性时，这些结果还表明这些体系是用于生物材料和医疗设备表面工程以调节药物释放的一个有前景的框架。
