Smart Materials, Istituto Italiano di Tecnologia, via Morego 30, 16163 Genoa, Italy; DIBRIS, University of Genoa, via Opera Pia 13, 16145 Genoa, Italy.
Smart Materials, Istituto Italiano di Tecnologia, via Morego 30, 16163 Genoa, Italy.
Acta Biomater. 2018 Jun;73:365-376. doi: 10.1016/j.actbio.2018.04.025. Epub 2018 Apr 17.
Human platelet lysate (hPL) is a pool of growth factors and cytokines able to induce regeneration of different tissues. Despite its good potentiality as therapeutic tool for regenerative medicine applications, hPL has been only moderately exploited in this field. A more widespread adoption has been limited because of its rapid degradation at room temperature that decreases its functionality. Another limiting factor for its extensive use is the difficulty of handling the hPL gels. In this work, silk fibroin-based patches were developed to address several points: improving the handling of hPL, enabling their delivery in a controlled manner and facilitating their storage by creating a device ready to use with expanded shelf life. Patches of fibroin loaded with hPL were synthesized by electrospinning to take advantage of the fibrous morphology. The release kinetics of the material was characterized and tuned through the control of fibroin crystallinity. Cell viability assays, performed with primary human dermal fibroblasts, demonstrated that fibroin is able to preserve the hPL biological activity and prolong its shelf-life. The strategy of storing and preserving small active molecules within a naturally-derived, protein-based fibrous scaffold was successfully implemented, leading to the design of a biocompatible device, which can potentially simplify the storage and the application of the hPL on a human patient, undergoing medical procedures such as surgery and wound care.
Human platelets lysate (hPL) is a mixture of growth factors and cytokines able to induce the regeneration of damaged tissues. This study aims at enclosing hPL in a silk fibroin electrospun matrix to expand its utilization. Silk fibroin showed the ability to preserve the hPL activity at temperature up to 60 °C and the manipulation of fibroin's crystallinity provided a tool to modulate the hPL release kinetic. This entails the possibility to fabricate the hPL silk fibroin patches in advance and store them, resulting in an easy and fast accessibility and an expanded use of hPL for wound healing.
人血小板裂解液（hPL）是一种生长因子和细胞因子的混合物，能够诱导不同组织的再生。尽管它作为再生医学应用的治疗工具具有良好的潜力，但在该领域的应用还只是中等程度。由于其在室温下迅速降解，降低了其功能，因此其更广泛的应用受到限制。另一个限制其广泛使用的因素是处理 hPL 凝胶的难度。在这项工作中，开发了基于丝素蛋白的贴片来解决几个问题：改善 hPL 的处理，以可控的方式进行输送，并通过创建一个准备好与扩展保质期一起使用的设备来方便其储存。通过静电纺丝合成了负载 hPL 的丝素蛋白贴片，以利用纤维形态。通过控制丝素蛋白结晶度来表征和调整材料的释放动力学。用原代人真皮成纤维细胞进行的细胞活力测定表明，丝素蛋白能够保持 hPL 的生物活性并延长其保质期。成功地将小分子活性物质储存在天然衍生的基于蛋白质的纤维支架内的策略，导致了设计出一种生物相容性装置，该装置有可能简化 hPL 在接受手术和伤口护理等医疗程序的患者中的储存和应用。
人血小板裂解液（hPL）是一种生长因子和细胞因子的混合物，能够诱导受损组织的再生。本研究旨在将 hPL 包埋在丝素蛋白静电纺丝基质中以扩大其用途。丝素蛋白显示出在高达 60°C 的温度下保持 hPL 活性的能力，并且控制丝素蛋白结晶度提供了一种调节 hPL 释放动力学的工具。这使得可以预先制造 hPL 丝素蛋白贴片并进行储存，从而实现了 hPL 用于伤口愈合的便捷和快速获取以及更广泛的应用。
