Legras B, Lemarchand I, Ruelland A, Gallou G, Cloarec L
Laboratoire de Biochemie A, Hôpital Sud, Rennes, France.
Pathol Biol (Paris). 1991 Oct;39(8):785-8.
A macrocreatine-kinase type 1 was identified by CK isoenzyme electrophoresis of a serum sample from a patient. Constituents of this enzyme complex were studied. The isoenzyme present in the macrocreatine-kinase was identified using immunoprecipitation with antibodies against the M subunit. Affinity chromatography then determined the type of the immunoglobulin bound to the CK isoenzyme. A protocol for studying macrocreatine-kinase type 1 compounds is proposed.
通过对一名患者血清样本进行肌酸激酶同工酶电泳，鉴定出一种1型巨肌酸激酶。对该酶复合物的成分进行了研究。使用针对M亚基的抗体进行免疫沉淀，鉴定了巨肌酸激酶中存在的同工酶。然后通过亲和层析确定与肌酸激酶同工酶结合的免疫球蛋白类型。提出了一种研究1型巨肌酸激酶化合物的方案。
