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[1型巨肌酸激酶的鉴定]

[Identification of a type 1 macrocreatine kinase].

作者信息

Legras B, Lemarchand I, Ruelland A, Gallou G, Cloarec L

机构信息

Laboratoire de Biochemie A, Hôpital Sud, Rennes, France.

出版信息

Pathol Biol (Paris). 1991 Oct;39(8):785-8.

PMID:1762837
Abstract

A macrocreatine-kinase type 1 was identified by CK isoenzyme electrophoresis of a serum sample from a patient. Constituents of this enzyme complex were studied. The isoenzyme present in the macrocreatine-kinase was identified using immunoprecipitation with antibodies against the M subunit. Affinity chromatography then determined the type of the immunoglobulin bound to the CK isoenzyme. A protocol for studying macrocreatine-kinase type 1 compounds is proposed.

摘要

通过对一名患者血清样本进行肌酸激酶同工酶电泳,鉴定出一种1型巨肌酸激酶。对该酶复合物的成分进行了研究。使用针对M亚基的抗体进行免疫沉淀,鉴定了巨肌酸激酶中存在的同工酶。然后通过亲和层析确定与肌酸激酶同工酶结合的免疫球蛋白类型。提出了一种研究1型巨肌酸激酶化合物的方案。

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