Kim Jayoung, Keay Susan K, Dimitrakov Jordan D, Freeman Michael R
The Urological Diseases Research Center, Children's Hospital Boston, Boston, MA 02115, USA.
FEBS Lett. 2007 Aug 7;581(20):3795-9. doi: 10.1016/j.febslet.2007.06.058. Epub 2007 Jul 2.
Antiproliferative factor (APF) is a sialoglycopeptide elevated in the urine of patients with interstitial cystitis, a urinary bladder disorder of unknown etiology that is characterized by chronic pelvic pain. The present study was directed toward uncovering a pathway through which APF signals. Treatment of human urothelial cells with native APF resulted in growth inhibition accompanied by blockade of cell cycle transit and increased p53. Reduced expression of p53 by RNA interference diminished, while ectopic expression of p53 mimicked, the effects of APF. These are the first findings implicating the network of p53 target genes in urothelial defects associated with interstitial cystitis.
抗增殖因子（APF）是一种唾液酸糖肽，在间质性膀胱炎患者的尿液中含量升高。间质性膀胱炎是一种病因不明的膀胱疾病，其特征为慢性盆腔疼痛。本研究旨在揭示APF发出信号的途径。用天然APF处理人膀胱上皮细胞会导致生长抑制，同时伴有细胞周期进程受阻和p53增加。通过RNA干扰降低p53的表达可减弱APF的作用，而p53的异位表达则模拟了APF的作用。这些是首次表明p53靶基因网络与间质性膀胱炎相关的膀胱上皮缺陷有关的发现。
