Kang BoKyu, Oh JinSik, Lee ChulSeung, Park Bong-Kyun, Park YoungNam, Hong KyungSoo, Lee KyungGi, Cho ByungKi, Song DaeSub
Research Unit, Green Cross Veterinary Products, Yongin 227-5, Kyunggi, South Korea.
J Virol Methods. 2007 Oct;145(1):30-6. doi: 10.1016/j.jviromet.2007.05.005. Epub 2007 Jul 12.
A rapid immunodiagnostic test kit for rabies virus detection was evaluated using 51 clinical samples and 4 isolates of rabies virus. The quick detection of rabies virus under field conditions may be helpful in determining if post-exposure prophylaxis is needed, thereby avoiding unnecessary treatments, as well as undue economic burden. There are several widely used diagnostic methods for rabies, including fluorescent antibody tests, reverse transcription polymerase chain reaction, and electron microscopy; however, these methods include time-consuming, intricate, and costly procedures. The rapid immunodiagnostic test was able to detect rabies virus in clinical samples, including brain tissue and saliva, in addition to 10(3.2) 50% lethal dose (LD(50))/mL cell-adapted rabies virus. The assay was not cross-reactive with non-rabies virus microbes. When the performance of the rapid immunodiagnostic test was compared to a fluorescent antibody test, the rapid immunodiagnostic test had a sensitivity of 91.7% and specificity of 100% (95.8% CI).
使用51份临床样本和4株狂犬病病毒分离株对一种用于检测狂犬病病毒的快速免疫诊断试剂盒进行了评估。在现场条件下快速检测狂犬病病毒可能有助于确定是否需要进行暴露后预防，从而避免不必要的治疗以及过度的经济负担。狂犬病有几种广泛使用的诊断方法，包括荧光抗体检测、逆转录聚合酶链反应和电子显微镜检查；然而，这些方法包括耗时、复杂且成本高昂的程序。这种快速免疫诊断测试能够在临床样本中检测狂犬病病毒，包括脑组织和唾液，此外还能检测到10(3.2) 50%致死剂量（LD(50)）/mL细胞适应狂犬病病毒。该检测方法与非狂犬病病毒微生物无交叉反应。当将快速免疫诊断测试的性能与荧光抗体检测进行比较时，快速免疫诊断测试的灵敏度为91.7%，特异性为100%（95.8%置信区间）。
