Mission Rabies, Tonca, Panjim, Goa 403002, India.
The Roslin Institute and The Royal (Dick) School of Veterinary Studies, Division of Genetics and Genomics, The University of Edinburgh, Easter Bush Veterinary Centre, Roslin, Midlothian EH25 9RG, UK.
Viruses. 2019 Jul 15;11(7):649. doi: 10.3390/v11070649.
Rabies is a fatal zoonotic disease transmitted by the bite of a rabid animal. More than 95% of the human rabies cases in India are attributed to exposure to rabid dogs. This study evaluated the utility of a lateral flow immunochromatographic assay (LFA) (Anigen Rapid Rabies Ag Test Kit, Bionote, Hwaseong-si, Korea) for rapid post mortem diagnosis of rabies in dogs. Brain tissue was collected from 202 animals that were screened through the Government of Goa rabies surveillance system. The brain tissue samples were obtained from 188 dogs, nine cats, three bovines, one jackal and one monkey. In addition, 10 dogs that died due to trauma from road accidents were included as negative controls for the study. The diagnostic performance of LFA was evaluated using results from direct fluorescence antibody test (dFT); the current gold standard post mortem test for rabies infection. Three samples were removed from the analysis as they were autolysed and not fit for testing by dFT. Of the 209 samples tested, 117 tested positive by LFA and 92 tested negative, while 121 tested positive by dFT and 88 tested negative. Estimates of LFA sensitivity and specificity were 0.96 (95% CI 0.91-0.99) and 0.99 (95% CI 0.94-1.00), respectively. The LFA is a simple and low-cost assay that aids in the rapid diagnosis of rabies in the field without the need for expensive laboratory equipment or technical expertise. This study found that Bionote LFA has potential as a screening tool in rabies endemic countries.
狂犬病是一种致命的人畜共患病，由患病动物的咬伤传播。在印度，超过 95%的人类狂犬病病例归因于接触狂犬病犬。本研究评估了侧向流动免疫层析测定法（LFA）（Anigen Rapid Rabies Ag Test Kit，Bionote，Hwaseong-si，Korea）在狗死后快速诊断狂犬病的实用性。从通过果阿邦狂犬病监测系统筛选的 202 只动物中采集脑组织样本。脑组织样本来自 188 只狗、9 只猫、3 只牛、1 只豺和 1 只猴子。此外，还纳入了 10 只因交通事故创伤而死亡的狗作为阴性对照。LFA 的诊断性能使用直接荧光抗体试验（dFT）的结果进行评估；dFT 是目前用于狂犬病感染的死后检测金标准。由于自溶且不适合 dFT 检测，从分析中删除了 3 个样本。在测试的 209 个样本中，117 个通过 LFA 检测呈阳性，92 个呈阴性，而 121 个通过 dFT 检测呈阳性，88 个呈阴性。LFA 的敏感性和特异性估计分别为 0.96（95%置信区间 0.91-0.99）和 0.99（95%置信区间 0.94-1.00）。LFA 是一种简单且低成本的检测方法，无需昂贵的实验室设备或技术专长，可在现场快速诊断狂犬病。本研究发现，Bionote LFA 有可能成为狂犬病流行国家的筛选工具。
