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一种使用双核铂配合物交联互补寡核苷酸的高效、序列特异性方法。

An efficient, sequence-specific method for crosslinking complementary oligonucleotides using binuclear platinum complexes.

作者信息

Gruff E S, Orgel L E

机构信息

Salk Institute for Biological Studies, San Diego, CA 92186-5800.

出版信息

Nucleic Acids Res. 1991 Dec 25;19(24):6849-54. doi: 10.1093/nar/19.24.6849.

DOI:10.1093/nar/19.24.6849
PMID:1762915
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC329319/
Abstract

The binuclear PtII complexes [(trans-Pt(NH3)2Cl)2 (NH2(CH2)nNH2)]Cl2 (n = 4, 5 or 6), crosslink oligodeoxynucleotide-5'-phosphorothioates rapidly, specifically and efficiently to complementary single-stranded oligodeoxynucleotide targets. In the case that we investigated in detail, the most abundant crosslink is formed to the G residue complementary to the 5'-terminal C residue of the phosphorothioate. Less efficient crosslinking occurs to many other residues of the target. The same PtII complexes also bring about crosslinking efficiently to the polypurine tract in triple-helical DNA.

摘要

双核铂（II）配合物[(反式-Pt(NH3)2Cl)2 (NH2(CH2)nNH2)]Cl2（n = 4、5或6）能快速、特异性且高效地将寡脱氧核苷酸-5'-硫代磷酸酯与互补的单链寡脱氧核苷酸靶标交联。在我们详细研究的案例中，最丰富的交联是与硫代磷酸酯5'-末端C残基互补的G残基形成的。与靶标的许多其他残基发生的交联效率较低。相同的铂（II）配合物也能有效地使三链DNA中的聚嘌呤序列发生交联。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6b9/329319/61dc735236ea/nar00104-0168-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6b9/329319/d2da94cf56c9/nar00104-0166-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6b9/329319/124ba7cf8854/nar00104-0167-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6b9/329319/61dc735236ea/nar00104-0168-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6b9/329319/d2da94cf56c9/nar00104-0166-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6b9/329319/124ba7cf8854/nar00104-0167-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a6b9/329319/61dc735236ea/nar00104-0168-a.jpg

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