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通过将模板与互补寡脱氧核苷酸的铂-硫醇衍生物交联来抑制DNA合成。

Inhibition of DNA synthesis by cross-linking the template to platinum-thiol derivatives of complementary oligodeoxynucleotides.

作者信息

Chu B C, Orgel L E

机构信息

Salk Institute for Biological Studies, San Diego, CA 92138.

出版信息

Nucleic Acids Res. 1989 Jun 26;17(12):4783-98. doi: 10.1093/nar/17.12.4783.

DOI:10.1093/nar/17.12.4783
PMID:2473441
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC318032/
Abstract

Unsubstituted oligodeoxynucleotides, or oligodeoxynucleotides linked to poly-(L)-lysine, when hybridized to a 322 base long template, did not inhibit the production of full length DNA copies by the Klenow fragment of E. coli DNA polymerase I. However, synthesis was inhibited if the cysteamine derivative of the same oligomer was cross-linked to the template via PtII. Truncated products were formed by termination of DNA synthesis a small number of bases upstream from the 5'-end of the cross-linked oligomer. AMV reverse transcriptase behaved similarly but was also slightly inhibited by the hybridized oligomer or its poly-lysine derivative.

摘要

未取代的寡脱氧核苷酸，或与聚（L）-赖氨酸相连的寡脱氧核苷酸，与一个322个碱基长的模板杂交时，不会抑制大肠杆菌DNA聚合酶I的Klenow片段产生全长DNA拷贝。然而，如果同一寡聚物的半胱胺衍生物通过PtII与模板交联，则合成会受到抑制。截短产物是通过在交联寡聚物5'-端上游少数几个碱基处终止DNA合成而形成的。禽成髓细胞瘤病毒逆转录酶表现出类似的行为，但也会受到杂交寡聚物或其聚赖氨酸衍生物的轻微抑制。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a23/318032/15c6f7951b2d/nar00129-0367-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a23/318032/1f4a595519d0/nar00129-0361-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a23/318032/22ee46ab581e/nar00129-0361-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a23/318032/4566f7f82103/nar00129-0362-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a23/318032/34aa01750bc6/nar00129-0363-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a23/318032/1045f4e9dd31/nar00129-0366-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a23/318032/15c6f7951b2d/nar00129-0367-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a23/318032/1f4a595519d0/nar00129-0361-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a23/318032/22ee46ab581e/nar00129-0361-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a23/318032/4566f7f82103/nar00129-0362-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a23/318032/34aa01750bc6/nar00129-0363-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a23/318032/1045f4e9dd31/nar00129-0366-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4a23/318032/15c6f7951b2d/nar00129-0367-a.jpg

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本文引用的文献

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Derivatization of unprotected polynucleotides.未保护的多核苷酸的衍生化
Nucleic Acids Res. 1983 Sep 24;11(18):6513-29. doi: 10.1093/nar/11.18.6513.
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Terminal transferase: use of the tailing of DNA and for in vitro mutagenesis.末端转移酶：用于DNA加尾及体外诱变。
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Catalysis of accurate poly(C)-directed synthesis of 3'-5'-linked oligoguanylates by Zn2+.锌离子催化以聚胞嘧啶为模板精确合成3'-5'连接的寡聚鸟苷酸。
优化PtII寡核苷酸硫代磷酸酯复合物与互补寡核苷酸交联的效率。
Nucleic Acids Res. 1990 Sep 11;18(17):5163-71. doi: 10.1093/nar/18.17.5163.
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Sequence-specific cleavage of single-stranded DNA: oligodeoxynucleotide-EDTA X Fe(II).单链DNA的序列特异性切割：寡聚脱氧核苷酸-乙二胺四乙酸×亚铁离子（II）
Proc Natl Acad Sci U S A. 1985 Feb;82(4):968-72. doi: 10.1073/pnas.82.4.968.
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Sequence-dependent termination of in vitro DNA synthesis by cis- and trans-diamminedichloroplatinum (II).顺式和反式二氯二氨铂（II）对体外DNA合成的序列依赖性终止
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Quantitative hybridization-arrest of mRNA in Xenopus oocytes using single-stranded complementary DNA or oligonucleotide probes.使用单链互补DNA或寡核苷酸探针在非洲爪蟾卵母细胞中进行mRNA的定量杂交抑制。
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Hybridization triggered cross-linking of deoxyoligonucleotides.杂交引发脱氧寡核苷酸的交联。
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