Heat inactivation of DNA ligase prior to electroporation increases transformation efficiency.
作者信息
Ymer S
机构信息
Medical Molecular Biology Group, John Curtin School of Medical Research, Australian National University, Canberra City, ACT.
出版信息
Nucleic Acids Res. 1991 Dec 25;19(24):6960. doi: 10.1093/nar/19.24.6960.
Abstract
摘要
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A simple treatment of DNA in a ligation mixture prior to electroporation improves transformation frequency.在电穿孔之前对连接混合物中的DNA进行简单处理可提高转化频率。
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本文引用的文献
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High voltage E. coli electro-transformation with DNA following ligation.连接后使用DNA进行高压大肠杆菌电转化。
Nucleic Acids Res. 1988 Dec 23;16(24):11820. doi: 10.1093/nar/16.24.11820.
2
High efficiency transformation of E. coli by high voltage electroporation.通过高压电穿孔实现大肠杆菌的高效转化。
Nucleic Acids Res. 1988 Jul 11;16(13):6127-45. doi: 10.1093/nar/16.13.6127.
3
High efficiency electroporation of ligated DNA into bacteria.将连接好的DNA高效电穿孔导入细菌。
Nucleic Acids Res. 1990 Oct 11;18(19):5912. doi: 10.1093/nar/18.19.5912.
4
High-efficiency electro-transformation of Escherichia coli with DNA from ligation mixtures.利用连接混合物中的DNA对大肠杆菌进行高效电转化。
Nucleic Acids Res. 1990 Mar 25;18(6):1653. doi: 10.1093/nar/18.6.1653.
5
Electroporation of bacteria: a general approach to genetic transformation.
Genet Eng (N Y). 1990;12:275-95. doi: 10.1007/978-1-4613-0641-2_14.
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