High-efficiency electro-transformation of Escherichia coli with DNA from ligation mixtures.
作者信息
Jacobs M, Wnendt S, Stahl U
机构信息
Technische Universitaet Berlin, Fachgebiet fuer Mikrobiologie und Genetik, FDR.
出版信息
Nucleic Acids Res. 1990 Mar 25;18(6):1653. doi: 10.1093/nar/18.6.1653.
Abstract
摘要
相似文献
1
High-efficiency electro-transformation of Escherichia coli with DNA from ligation mixtures.利用连接混合物中的DNA对大肠杆菌进行高效电转化。
Nucleic Acids Res. 1990 Mar 25;18(6):1653. doi: 10.1093/nar/18.6.1653.
2
A new method to obtain high DNA transformation efficiency of E. coli competent cells.一种获得大肠杆菌感受态细胞高DNA转化效率的新方法。
Nucleic Acids Res. 1988 Sep 12;16(17):8727. doi: 10.1093/nar/16.17.8727.
3
Electro-stimulated transformation of E. coli cells pretreated by EDTA solution.经乙二胺四乙酸（EDTA）溶液预处理的大肠杆菌细胞的电刺激转化
FEBS Lett. 1988 Jul 4;234(1):203-7. doi: 10.1016/0014-5793(88)81334-6.
4
[Effect of the molecular weight of DNA on the effectiveness of plasmid transformation of E. coli K12].[DNA分子量对大肠杆菌K12质粒转化效率的影响]
Mol Gen Mikrobiol Virusol. 1988 Feb(2):39-41.
5
A simple treatment of DNA in a ligation mixture prior to electroporation improves transformation frequency.在电穿孔之前对连接混合物中的DNA进行简单处理可提高转化频率。
Nucleic Acids Res. 1993 Jun 11;21(11):2782. doi: 10.1093/nar/21.11.2782.
6
Low cost device for electrotransformation and its application to the highly efficient transformation of Escherichia coli and Enterococcus faecalis.用于电转化的低成本装置及其在大肠杆菌和粪肠球菌高效转化中的应用。
Plasmid. 1991 Sep;26(2):131-5. doi: 10.1016/0147-619x(91)90053-y.
7
Transformation of Escherichia coli increases 260-fold upon inactivation of T4 DNA ligase.在T4 DNA连接酶失活后，大肠杆菌的转化率增加260倍。
Anal Biochem. 1995 Feb 10;225(1):172-4. doi: 10.1006/abio.1995.1130.
8
High voltage E. coli electro-transformation with DNA following ligation.连接后使用DNA进行高压大肠杆菌电转化。
Nucleic Acids Res. 1988 Dec 23;16(24):11820. doi: 10.1093/nar/16.24.11820.
9
High efficiency 5 min transformation of Escherichia coli.大肠杆菌的高效5分钟转化
Nucleic Acids Res. 1996 Feb 1;24(3):536-7. doi: 10.1093/nar/24.3.536.
10
[In vivo restriction of Escherichia coli-transforming DNA by endonuclease R.M.EcoRI].[体内内切核酸酶R.M.EcoRI对大肠杆菌转化DNA的限制作用]
Genetika. 1981;17(7):1205-10.
引用本文的文献
1
A Review on Mycobacteriophages: From Classification to Applications.分枝杆菌噬菌体综述：从分类到应用
Pathogens. 2022 Jul 7;11(7):777. doi: 10.3390/pathogens11070777.
2
Mechanism of transformation in Mycobacteria using a novel shockwave assisted technique driven by in-situ generated oxyhydrogen.利用原位生成的氢氧气体驱动的新型冲击波辅助技术在分枝杆菌中实现转化的机制。
Sci Rep. 2017 Aug 17;7(1):8645. doi: 10.1038/s41598-017-08542-5.
3
A simple treatment of DNA in a ligation mixture prior to electroporation improves transformation frequency.在电穿孔之前对连接混合物中的DNA进行简单处理可提高转化频率。
Nucleic Acids Res. 1993 Jun 11;21(11):2782. doi: 10.1093/nar/21.11.2782.
4
Desiccation tolerance of prokaryotes.原核生物的耐旱性。
Microbiol Rev. 1994 Dec;58(4):755-805. doi: 10.1128/mr.58.4.755-805.1994.
5
High efficiency electroporation of ligated DNA into bacteria.将连接好的DNA高效电穿孔导入细菌。
Nucleic Acids Res. 1990 Oct 11;18(19):5912. doi: 10.1093/nar/18.19.5912.
6
Transformation of freshwater and marine caulobacters by electroporation.通过电穿孔法对淡水柄杆菌和海洋柄杆菌进行转化。
J Bacteriol. 1991 Jan;173(2):921-5. doi: 10.1128/jb.173.2.921-925.1991.
7
Heat inactivation of DNA ligase prior to electroporation increases transformation efficiency.在电穿孔之前对DNA连接酶进行热失活可提高转化效率。
Nucleic Acids Res. 1991 Dec 25;19(24):6960. doi: 10.1093/nar/19.24.6960.
本文引用的文献
1
Electroporation of eukaryotes and prokaryotes: a general approach to the introduction of macromolecules into cells.真核生物和原核生物的电穿孔：将大分子导入细胞的通用方法。
Biotechniques. 1988 Sep;6(8):742-51.
2
High efficiency transformation of E. coli by high voltage electroporation.通过高压电穿孔实现大肠杆菌的高效转化。
Nucleic Acids Res. 1988 Jul 11;16(13):6127-45. doi: 10.1093/nar/16.13.6127.
3
"ATG vectors' for regulated high-level expression of cloned genes in Escherichia coli.用于在大肠杆菌中调控克隆基因高水平表达的“ATG载体”。
Gene. 1985;40(2-3):183-90. doi: 10.1016/0378-1119(85)90041-1.
Zotero 插件