High voltage E. coli electro-transformation with DNA following ligation.
作者信息
Willson T A, Gough N M
机构信息
Walter and Eliza Hall Institute of Medical Research, Melbourne, Victoria, Australia.
出版信息
Nucleic Acids Res. 1988 Dec 23;16(24):11820. doi: 10.1093/nar/16.24.11820.
Abstract
摘要
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Nucleic Acids Res. 1988 Dec 23;16(24):11820. doi: 10.1093/nar/16.24.11820.
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引用本文的文献
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A simple treatment of DNA in a ligation mixture prior to electroporation improves transformation frequency.在电穿孔之前对连接混合物中的DNA进行简单处理可提高转化频率。
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High efficiency electroporation of ligated DNA into bacteria.将连接好的DNA高效电穿孔导入细菌。
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PCR based gene engineering of the Vibrio harveyi lux operon and the Escherichia coli trp operon provides for biochemically functional native and fused gene products.基于聚合酶链反应（PCR）对哈维氏弧菌lux操纵子和大肠杆菌trp操纵子进行基因工程改造，可产生具有生化功能的天然和融合基因产物。
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Heat inactivation of DNA ligase prior to electroporation increases transformation efficiency.在电穿孔之前对DNA连接酶进行热失活可提高转化效率。
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Sequence diversity among related genes for recognition of specific targets in DNA molecules.用于识别DNA分子中特定靶标的相关基因间的序列多样性。
J Mol Biol. 1983 May 5;166(1):1-19. doi: 10.1016/s0022-2836(83)80047-3.
3
High efficiency transformation of E. coli by high voltage electroporation.通过高压电穿孔实现大肠杆菌的高效转化。
Nucleic Acids Res. 1988 Jul 11;16(13):6127-45. doi: 10.1093/nar/16.13.6127.
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