Strauch Berish, Patel Mitesh K, Navarro Jose A, Berdichevsky Max, Yu Han-Liang, Pilla Arthur A
New York, N.Y. From the Department of Plastic and Reconstructive Surgery, Albert Einstein College of Medicine and Montefiore Medical Center; Department of Biomedical Engineering, Columbia University; and Department of Orthopedics, Mount Sinai School of Medicine.
Plast Reconstr Surg. 2007 Aug;120(2):425-430. doi: 10.1097/01.prs.0000267700.15452.d0.
Previous studies of pulsed magnetic fields have reported enhanced fracture and chronic wound healing, endothelial cell growth, and angiogenesis. This study characterizes the biomechanical changes that occur when standard cutaneous wounds are exposed to radiofrequency pulsed magnetic fields with specific dosage parameters, in an attempt to determine whether return to functional tensile strength could be accelerated in wound healing.
There were two study phases and a total of 100 rats. In phase 1, wounds were exposed to a 1.0-G pulsed magnetic field signal in clinical use for wound repair for 30 minutes twice daily for 21 or 60 days. Phase 2 was a prospective, placebo-controlled, double-blind trial in which rats were treated for 30 minutes twice daily with three different low-amplitude signals (0.02 to 0.05 G), configured assuming a Ca binding transduction pathway, for 21 days. A midline, 8-cm, linear skin incision was made on the rat dorsum. Tensile strength was determined by measuring the point of rupture of the wound on a standard tensiometer loaded at 0.45 mm/second.
The mean tensile strength of treated groups in phase 1 was 48 percent (p < 0.001) greater than that of controls at 21 days; there was no significant difference at 60 days. In phase 2, the treated groups showed 18 percent (not significant), 44 percent, and 59 percent (p < 0.001) increases in tensile strength over controls at 21 days.
The authors successfully demonstrated that exposing wounds to pulsed magnetic fields of very specific configurations accelerated early wound healing in this animal model, as evidenced by significantly increased wound tensile strength at 21 days after wounding.
先前关于脉冲磁场的研究报告称，其可促进骨折愈合、慢性伤口愈合、内皮细胞生长及血管生成。本研究旨在确定当标准皮肤伤口暴露于具有特定剂量参数的射频脉冲磁场时所发生的生物力学变化，以试图判断伤口愈合过程中恢复至功能拉伸强度的速度是否能够加快。
本研究分为两个阶段，共有100只大鼠。在第一阶段，伤口暴露于临床用于伤口修复的1.0 G脉冲磁场信号下，每天两次，每次30分钟，持续21天或60天。第二阶段是一项前瞻性、安慰剂对照、双盲试验，大鼠每天两次接受三种不同低振幅信号（0.02至0.05 G）治疗，每次30分钟，信号配置假设为钙结合转导途径，持续21天。在大鼠背部做一个8厘米长的中线线性皮肤切口。通过在以0.45毫米/秒加载的标准张力计上测量伤口的破裂点来确定拉伸强度。
在第1阶段，治疗组在21天时的平均拉伸强度比对照组高48%（p < 0.001）；在60天时无显著差异。在第2阶段，治疗组在21天时的拉伸强度比对照组分别增加了18%（无显著性差异）、44%和59%（p < 0.001）。
作者成功证明，在该动物模型中，将伤口暴露于非常特定配置的脉冲磁场可加速早期伤口愈合，受伤后21天时伤口拉伸强度显著增加即证明了这一点。
