使用实时聚合酶链反应分析法对银屑病患者鳞屑中的马拉色菌进行定量分析。
Quantitative analysis of Malassezia in the scale of patients with psoriasis using a real-time polymerase chain reaction assay.
作者信息
Takahata Y, Sugita T, Hiruma M, Muto M
机构信息
Department of Dermatology, Yamaguchi University Graduate School of Medicine, Ube, Yamaguchi, Japan.
出版信息
Br J Dermatol. 2007 Oct;157(4):670-3. doi: 10.1111/j.1365-2133.2007.08090.x. Epub 2007 Jul 19.
BACKGROUND
It has been suggested that Malassezia is associated with the development of skin lesions in psoriasis because of the response of the scalp lesions in psoriasis to antifungal agents. Malassezia restricta and M. globosa are the two major members of the cutaneous Malassezia flora in patients with psoriasis, although they have not been analysed quantitatively.
OBJECTIVES
This study quantified the two major cutaneous Malassezia species in psoriatic scale from different body sites using a real-time polymerase chain reaction (PCR) assay.
METHODS
Scale samples were collected from lesional and nonlesional skin of 20 Japanese patients with psoriasis and fungal DNA was extracted from the samples directly. All the Malassezia species, including the two major species M. globosa and M. restricta, were quantified with high accuracy, using a real-time PCR assay.
RESULTS
Colonization by M. restricta was approximately five times higher at all body sites than colonization by M. globosa. Malassezia colonization was significantly lower in patients with hyperlipidaemia than in patients with normolipidaemia.
CONCLUSIONS
Malassezia restricta is the predominant species in psoriatic scale.
背景
由于银屑病头皮损害对抗真菌药物有反应，有人提出马拉色菌与银屑病皮肤损害的发生有关。虽然尚未进行定量分析，但限制马拉色菌和球形马拉色菌是银屑病患者皮肤马拉色菌群的两个主要成员。
目的
本研究使用实时聚合酶链反应（PCR）分析法对来自不同身体部位的银屑病鳞屑中两种主要的皮肤马拉色菌进行定量。
方法
收集20例日本银屑病患者皮损和非皮损皮肤的鳞屑样本，并直接从样本中提取真菌DNA。使用实时PCR分析法对所有马拉色菌种，包括两个主要菌种球形马拉色菌和限制马拉色菌进行高精度定量。
结果
在所有身体部位，限制马拉色菌的定植率比球形马拉色菌高约五倍。高脂血症患者的马拉色菌定植率明显低于血脂正常的患者。
结论
限制马拉色菌是银屑病鳞屑中的优势菌种。
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