Wicking C, Williamson B
Department of Biochemistry and Molecular Genetics, St Mary's Hospital, Medical School, London, UK.
Trends Genet. 1991 Sep;7(9):288-93. doi: 10.1016/0168-9525(91)90310-M.
An area of increasing importance in human genetics research is the strategy of 'reverse genetics' or 'positional cloning', whereby a gene associated with a genetic disease is isolated on the basis of its approximate chromosomal position. This article and the accompanying centrepage diagram describe the main features of this strategy and outline the techniques involved; they are designed to be used for teaching purposes and as a general reference for those from other fields of genetics research.
在人类遗传学研究中，一个日益重要的领域是“反向遗传学”或“定位克隆”策略，即根据与遗传疾病相关基因的大致染色体位置来分离该基因。本文及随附的中页图表描述了这一策略的主要特征，并概述了所涉及的技术；它们旨在用于教学目的，并作为遗传学研究其他领域人员的一般参考资料。
