Eutamene Hélène, Lamine Florence, Chabo Chantal, Theodorou Vassilia, Rochat Florence, Bergonzelli Gabriela E, Corthésy-Theulaz Irène, Fioramonti Jean, Bueno Lionel
Neuro-Gastroenterology and Nutrition Unit, UMR 1054 INRA/ EI-Purpan, Toulouse, France.
J Nutr. 2007 Aug;137(8):1901-7. doi: 10.1093/jn/137.8.1901.
Stressful events result in the alteration of gut permeability and sensitivity. Lactobacillus paracasei NCC2461 (Lpa) therapy prevents antibiotic-induced visceral hyperalgesia in mice. This study aimed at evaluating the influence of 3 probiotic strains: Bifidobacterium lactis NCC362, Lactobacillus johnsonii NCC533, and Lpa on stress-mediated alterations of colorectal hyperalgesia, on gut paracellular permeability and whether bacteria and/or bacterial products present in the spent culture medium (SCM) were involved in the antinociceptive properties of the effective strain. Rat pups were separated from their mothers 3 h/d during postnatal d 2-14. At wk 13, gut paracellular permeability was determined as a percentage of urinary excreted (51)Cr-EDTA and visceral sensitivity to colorectal distension (CRD), assessed by abdominal muscle electromyography. Visceral sensitivity was also analyzed in adults rats subjected to partial restraint stress (PRS, 2 h restriction of body movements). Rats received either the probiotics resuspended in SCM or fresh growth medium as control for 2 wk. Maternal deprivation significantly increased colonic sensitivity in response to CRD and enhanced gut paracellular permeability compared with control rats. Only Lpa treatment significantly improved stress-induced visceral pain and restored normal gut permeability. Similarly, among the 3 probiotics tested, only Lpa prevented PRS-mediated visceral hyperalgesia. Both bacteria and bacterial products present in Lpa SCM were required for the antinociceptive properties against PRS. This study illustrates strain-specific effects and suggests a synergistic interplay between L. paracasei bacteria and bacterial products generated during fermentation and growth that confers the ability to suppress PRS-induced hypersensitivity in rats.
应激事件会导致肠道通透性和敏感性发生改变。副干酪乳杆菌NCC2461（Lpa）疗法可预防小鼠抗生素诱导的内脏痛觉过敏。本研究旨在评估3种益生菌菌株：乳酸双歧杆菌NCC362、约氏乳杆菌NCC533和Lpa对应激介导的结直肠痛觉过敏改变、肠道细胞旁通透性的影响，以及废培养基（SCM）中存在的细菌和/或细菌产物是否参与有效菌株的抗伤害感受特性。在出生后第2至14天，将幼鼠每天与母亲分离3小时。在第13周时，通过测定尿中排出的（51）铬-乙二胺四乙酸（51）Cr-EDTA的百分比来确定肠道细胞旁通透性，并通过腹部肌肉肌电图评估对结直肠扩张（CRD）的内脏敏感性。还对遭受部分束缚应激（PRS，身体活动限制2小时）的成年大鼠的内脏敏感性进行了分析。大鼠接受重悬于SCM中的益生菌或新鲜生长培养基作为对照，持续2周。与对照大鼠相比，母婴分离显著增加了对CRD的结肠敏感性，并增强了肠道细胞旁通透性。只有Lpa治疗显著改善了应激诱导的内脏疼痛并恢复了正常的肠道通透性。同样，在测试的3种益生菌中，只有Lpa预防了PRS介导的内脏痛觉过敏。Lpa SCM中存在的细菌和细菌产物对于抵抗PRS的抗伤害感受特性都是必需的。本研究说明了菌株特异性效应，并表明副干酪乳杆菌与发酵和生长过程中产生的细菌产物之间存在协同相互作用，这种相互作用赋予了抑制大鼠PRS诱导的超敏反应的能力。
