Pratt McKenna L, Plumb Ashley N, Manjrekar Aditi, Cardona Lucia M, Chan Cheri K, John Juanna M, Sadler Katelyn E
Department of Neuroscience, Center for Advanced Pain Studies, University of Texas at Dallas, Richardson, TX, United States.
Pain. 2025 Feb 1;166(2):262-281. doi: 10.1097/j.pain.0000000000003376. Epub 2024 Sep 10.
Over the past 2 decades, the microbiome has received increasing attention for the role that it plays in health and disease. Historically, the gut microbiome was of particular interest to pain scientists studying nociplastic visceral pain conditions given the anatomical juxtaposition of these microorganisms and the neuroimmune networks that drive pain in such diseases. More recently, microbiomes both inside and across the surface of the body have been recognized for driving sensory symptoms in a broader set of diseases. Microbiomes have never been a more popular topic in pain research, but to date, there has not been a systematic review of the preclinical microbiome pain literature. In this article, we identified all animal studies in which both the microbiome was manipulated and pain behaviors were measured. Our analysis included 303 unique experiments across 97 articles. Microbiome manipulation methods and behavioral outcomes were recorded for each experiment so that field-wide trends could be quantified and reported. This review specifically details the animal species, injury models, behavior measures, and microbiome manipulations used in preclinical pain research. From this analysis, we were also able to conclude how manipulations of the microbiome alter pain thresholds in naïve animals and persistent pain intensity and duration in cutaneous and visceral pain models. This review summarizes by identifying existing gaps in the literature and providing recommendations for how to best plan, implement, and interpret data collected in preclinical microbiome pain experiments.
在过去的20年里，微生物群因其在健康和疾病中所起的作用而受到越来越多的关注。从历史上看，肠道微生物群对研究神经病理性内脏疼痛状况的疼痛科学家来说特别有吸引力，因为这些微生物与驱动此类疾病疼痛的神经免疫网络在解剖学上相邻。最近，体内和体表的微生物群被认为在更广泛的一组疾病中引发感觉症状。微生物群在疼痛研究中从未像现在这样热门，但迄今为止，尚未对临床前微生物群疼痛文献进行系统综述。在本文中，我们识别了所有既对微生物群进行了操控又测量了疼痛行为的动物研究。我们的分析包括来自97篇文章的303个独特实验。记录了每个实验的微生物群操控方法和行为结果，以便能够量化和报告整个领域的趋势。本综述具体详细介绍了临床前疼痛研究中使用的动物物种、损伤模型、行为测量方法和微生物群操控方法。通过这一分析，我们还能够得出微生物群操控如何改变未受过处理的动物的疼痛阈值以及皮肤和内脏疼痛模型中持续性疼痛的强度和持续时间。本综述通过识别文献中的现有空白并就如何最好地规划、实施和解释临床前微生物群疼痛实验中收集的数据提供建议进行总结。
