McShine R L, Das P C, Sibinga C T, Brozović B
Red Cross Blood Bank Groningen-Drenthe, Groningen, The Netherlands.
Vox Sang. 1991;61(2):84-9. doi: 10.1111/j.1423-0410.1991.tb00251.x.
We have shown in this study that addition of dried K2EDTA (1.5 mg/ml) to blood samples anticoagulated with CPDA-1 increases significantly the platelet count and mean platelet volume (MPV) of whole blood, red cell concentrate (RCC) and platelet concentrate (PC), but not of platelet-rich plasma (PRP) or of platelet-poor plasma (PPP). Transmission and scanning electron microscopy illustrated that platelet aggregates which are present in some components are dispersed on mixing of the sample with EDTA and that this is accompanied by a change in platelet morphology. Determination of the platelet distribution width (PDW) indicated that the platelet populations in whole blood and RCC seem to be more uniform in size than the populations in PRP, PPP and PC. The determination of MPV and PDW changes after addition of EDTA may provide a new approach to quality control of PC.
我们在本研究中表明，向用CPDA-1抗凝的血样中添加干燥的K2EDTA（1.5毫克/毫升）可显著提高全血、红细胞浓缩物（RCC）和血小板浓缩物（PC）的血小板计数和平均血小板体积（MPV），但对富血小板血浆（PRP）或贫血小板血浆（PPP）则无此作用。透射电子显微镜和扫描电子显微镜显示，某些成分中存在的血小板聚集体在样品与EDTA混合时会分散，并且这伴随着血小板形态的变化。血小板分布宽度（PDW）的测定表明，全血和RCC中的血小板群体在大小上似乎比PRP、PPP和PC中的群体更均匀。添加EDTA后MPV和PDW变化的测定可能为PC的质量控制提供一种新方法。
