Stephenson Emma L, Braude Peter R, Mason Chris
Advanced Center for Biochemical Engineering, University College London, London, UK.
Regen Med. 2007 Jul;2(4):349-62. doi: 10.2217/17460751.2.4.349.
The development and implementation of new methods in human embryonic stem cell (hESC) research is fraught with difficulties, not least of which is the highly variable reporting of the number and quality of embryos used to derive hESC lines. Without a clear minimum information convention among the derivation teams, understanding and comparing derivation methods and their potential impact on the resulting stem cell line will continue to be extremely difficult. In order to address this issue, we consulted international teams regarding the implementation of a minimum information convention for derivation with the aim of universal use, data collection and central analysis, followed by a multi-author publication. The responses demonstrated overwhelming support for such a system; over 90% of the respondents agreed that a universal standard for reporting the derivation of hESC lines was essential as part of the international effort to advance the field efficiently, and over 87% plan to use this standard and share collected data in Spring 2008 for central analysis and public dissemination. A number of future steps are planned in order to ensure that this standard evolves with the field and remains relevant and up-to-date. Our aim is to incorporate these data within existing international initiatives aimed at improving derivation standards. This article is an open-access publication in order to make the convention freely available to the international community and encourage universal participation.
人类胚胎干细胞（hESC）研究中新方法的开发与实施充满困难，其中最主要的困难之一是用于建立hESC系的胚胎数量和质量的报告差异很大。由于在建立细胞系的团队之间没有明确的最低信息规范，理解和比较建立细胞系的方法及其对所得干细胞系的潜在影响将继续极其困难。为了解决这个问题，我们就实施一个用于细胞系建立的最低信息规范向国际团队进行了咨询，目的是实现普遍使用、数据收集和集中分析，随后进行多作者发表。反馈显示对此类系统的大力支持；超过90%的受访者认为，作为高效推动该领域发展的国际努力的一部分，报告hESC系建立情况的通用标准至关重要，超过87%的受访者计划在2008年春季使用该标准并分享收集的数据，以供集中分析和公开传播。为确保该标准能随该领域发展而不断完善并始终保持相关性和时效性，还计划了一些后续步骤。我们的目标是将这些数据纳入旨在提高细胞系建立标准的现有国际倡议中。本文是一篇开放获取出版物，以便国际社会能够免费获取该规范并鼓励广泛参与。
