Department of Biomedical and Biotechnological Sciences, University of Catania, Catania, Italy.
Reproductive and IVF Unit- PTA "F Jaia", Department of Maternal and Child Health, Conversano, Italy.
Sci Rep. 2019 Jan 14;9(1):84. doi: 10.1038/s41598-018-36452-7.
In this study, for the first time, we demonstrated the presence of microRNAs and extracellular vesicles in human blastocoel fluid. The bioinformatic and comparative analyses identified the biological function of blastocoel fluid microRNAs and suggested a potential role inside the human blastocyst. We found 89 microRNAs, expressed at different levels, able to regulate critical signaling pathways controlling embryo development, such as pluripotency, cell reprogramming, epigenetic modifications, intercellular communication, cell adhesion and cell fate. Blastocoel fluid microRNAs reflect the miRNome of embryonic cells and their presence, associated with the discovery of extracellular vesicles, inside blastocoel fluid, strongly suggests their important role in mediating cell communication among blastocyst cells. Their characterization is important to better understand the earliest stages of embryogenesis and the complex circuits regulating pluripotency. Moreover, blastocoel fluid microRNA profiles could be influenced by blastocyst quality, therefore, microRNAs might be used to assess embryo potential in IVF cycles.
在这项研究中，我们首次证明了 microRNAs 和细胞外囊泡存在于人类囊胚腔液中。生物信息学和比较分析确定了囊胚腔液 microRNAs 的生物学功能，并提示了它们在人类囊胚中的潜在作用。我们发现了 89 种 microRNAs，它们以不同的水平表达，能够调节控制胚胎发育的关键信号通路，如多能性、细胞重编程、表观遗传修饰、细胞间通讯、细胞黏附和细胞命运。囊胚腔液 microRNAs 反映了胚胎细胞的 miRNome，它们的存在与细胞外囊泡的发现一起，强烈提示它们在介导囊胚细胞间的细胞通讯中发挥着重要作用。对它们进行特征分析对于更好地理解胚胎发生的最早阶段和调节多能性的复杂回路非常重要。此外，囊胚腔液 microRNA 谱可能受到囊胚质量的影响，因此，microRNAs 可能用于评估体外受精周期中的胚胎潜能。
