Michels J
Alfred-Wegener-Institut für Polar- und Meeresforschung, PO Box 120161, 27515 Bremerhaven, Germany.
J Microsc. 2007 Jul;227(Pt 1):1-7. doi: 10.1111/j.1365-2818.2007.01787.x.
The utility of cuticular autofluorescence for the visualization of copepod morphology by means of confocal laser scanning microscopy (CLSM) was examined. Resulting maximum intensity projections give very accurate information on morphology and show even diminutive structures such as small setae in detail. Furthermore, CLSM enables recognition of internal structures and differences in material composition. Optical sections in all layers and along all axes of the specimens can be obtained by CLSM. The facile and rapid preparation method bears no risk of artefacts or damage occurring to the preparations and the visualized specimens can be used for later analyses allowing for the investigation of irreplaceable type specimens or parts of them. These features make CLSM a very effective tool for both taxonomical and ecological studies in small crustaceans; however, the maximum thickness of the specimens is limited to a few hundred micrometers. Three-dimensional models based on CLSM image stacks allow observation of the preparations from all angles and can permit, improve and speed up studies on functional morphology. The visualization method described has a strong potential to become a future standard technique in aquatic biology due to its advantages over conventional light microscopy and scanning electron microscopy.
研究了通过共聚焦激光扫描显微镜（CLSM）利用表皮自发荧光来观察桡足类形态的效用。所得的最大强度投影能提供非常准确的形态信息，甚至能详细显示微小的结构，如小刚毛。此外，CLSM能够识别内部结构以及材料成分的差异。通过CLSM可以获得标本所有层面和所有轴向上的光学切片。这种简便快速的制备方法不会对标本造成人为假象或损坏风险，并且可视化的标本可用于后续分析，从而能够研究不可替代的模式标本或其部分。这些特性使CLSM成为小型甲壳类动物分类学和生态学研究的非常有效的工具；然而，标本的最大厚度限制在几百微米。基于CLSM图像堆栈的三维模型允许从各个角度观察标本，并且可以促进、改进和加速功能形态学研究。由于其相对于传统光学显微镜和扫描电子显微镜的优势，所描述的可视化方法很有可能成为水生生物学未来的标准技术。
