Lademann Juergen, Otberg Nina, Richter Heike, Meyer Lars, Audring Heike, Teichmann Alexa, Thomas Steven, Knüttel Alexander, Sterry Wolfram
Universitätsmedizin Charité Berlin, Department of Dermatology and Allergy, Center of Experimental and Applied Cutaneous Physiology (CCP), Germany.
Skin Res Technol. 2007 May;13(2):119-32. doi: 10.1111/j.1600-0846.2007.00208.x.
BACKGROUND/PURPOSE: Optical, non-invasive methods, such as fluorescence laser scanning microscopy (LSM) and optical coherent tomography (OCT), have become efficient tools for the characterization of the skin structure in vivo, as well as real-time investigation of distribution and penetration of topically applied substances.
In the present paper, the results obtained with both non-invasive methods - OCT and LSM - were compared to conventional light microscopy of histological sections. Skin structure and the distribution of topically applied particulate and non-particulate substances on the skin surface and in the epidermis were analyzed.
None of the methods used are suitable for the realization of all diagnostic tasks, however, each method has advantages for particular applications. Fluorescence LSM is well suited for the investigation of the upper 150 microm of the skin as well as for the investigation of the kinetics of substances applied onto or into the epidermis. OCT can be applied for the investigation of vertical cross-sections of the skin up to a depth of 2 mm, albeit at lower resolution than achieved by LSM or conventional light microscopy. Conventional light microscopy of histological sections of biopsy specimens produces familiar high-resolution images of deeper tissue layers. However, the analysis of the kinetic processes is limited in this case.
LSM- and OCT-measurements are efficient non-invasive tools for the characterization of morphological structures of the skin. On the one hand, the optical methods have a clear advantage in the case of kinetic measurements. On the other hand, histological investigations are characterized by a high information density and a high resolution, also in deep tissue layers. The selection of the best method for the analysis of the skin morphology depends on the target and the task of the investigation.
背景/目的：光学非侵入性方法，如荧光激光扫描显微镜（LSM）和光学相干断层扫描（OCT），已成为体内皮肤结构表征以及局部应用物质分布和渗透实时研究的有效工具。
在本文中，将非侵入性方法OCT和LSM所获得的结果与组织学切片的传统光学显微镜检查结果进行了比较。分析了皮肤结构以及局部应用的颗粒和非颗粒物质在皮肤表面和表皮中的分布。
所使用的方法均不适用于完成所有诊断任务，然而，每种方法在特定应用中都有其优势。荧光LSM非常适合研究皮肤上部150微米以及应用于表皮上或表皮内的物质的动力学。OCT可用于研究深度达2毫米的皮肤垂直横截面，尽管其分辨率低于LSM或传统光学显微镜。活检标本组织学切片的传统光学显微镜检查可产生熟悉的深层组织高分辨率图像。然而，在这种情况下，动力学过程的分析受到限制。
LSM和OCT测量是表征皮肤形态结构的有效非侵入性工具。一方面，光学方法在动力学测量方面具有明显优势。另一方面，组织学研究的特点是信息密度高且分辨率高，在深层组织中也是如此。选择最佳的皮肤形态分析方法取决于研究的目标和任务。
