Coticchia J M, Heiselman F A, Gharbo R, DeMaria T F, Lim D J
Otological Research Laboratories, Ohio State University College of Medicine, Columbus 43210.
Acta Otolaryngol. 1991;111(6):1097-104. doi: 10.3109/00016489109100762.
It has been demonstrated that the eustachian tube and middle ear epithelium produce Tubal Surface Active Substances (TSAS), which facilitate the opening of the eustachian tube. In order to characterize the biochemical contents of chinchilla TSAS, the tubal washings were analyzed using 2-D thin layer chromatography. The results indicate that phosphatidylcholine was the predominant phospholipid, followed by sphingomyelin, phosphatidylinositol phosphatidylethanolamine, and phosphatidylserine. In comparison, pulmonary lavage showed phosphatidylcholine to be highest allowed by phosphatidylethanolamine and sphingomyelin. Phosphatidylcholine/phosphatidylethanolanim ratios were 5:1 in the tubal lavage, and 8:1 in the pulmonary lavage. Phosphatidylcholine/sphingomyelin ratios were 2:1 in the tubal lavage, and 67:1 in the pulmonary lavage. It is concluded that the biochemical content of TSAS is similar but not identical to that of pulmonary surfactants.
已证实咽鼓管和中耳上皮会产生咽鼓管表面活性物质（TSAS），其有助于咽鼓管开放。为了表征栗鼠TSAS的生化成分，采用二维薄层色谱法对咽鼓管冲洗液进行了分析。结果表明，磷脂酰胆碱是主要的磷脂，其次是鞘磷脂、磷脂酰肌醇、磷脂酰乙醇胺和磷脂酰丝氨酸。相比之下，肺灌洗显示磷脂酰胆碱含量最高，其次是磷脂酰乙醇胺和鞘磷脂。咽鼓管灌洗中磷脂酰胆碱/磷脂酰乙醇胺的比例为5:1，肺灌洗中为8:1。咽鼓管灌洗中磷脂酰胆碱/鞘磷脂的比例为2:1，肺灌洗中为67:1。得出的结论是，TSAS的生化成分与肺表面活性剂相似但并不相同。
