Lardeux Frédéric, Quispe Vicente, Tejerina Rosenka, Rodríguez Roberto, Torrez Libia, Bouchité Bernard, Chávez Tamara
Institut de recherche pour le développement (IRD), UR Caractérisation et contrôle des populations de vecteurs, C.P. 9214, La Paz, Bolivia.
C R Biol. 2007 Aug;330(8):571-5. doi: 10.1016/j.crvi.2007.04.002. Epub 2007 May 9.
Anopheles pseudopunctipennis is one of the main malaria vectors in the Andean regions of South America. Few experimental data exist on this species because it is not very available in laboratories due to its eurygamic status that makes colony maintenance difficult. Indeed, individuals do not mate in the confined space of insectary cages. To avoid this problem, forced artificial mating can be used. However, this technique is time consuming, requires a well-trained technician, and is inadequate for easy mass production, which is sometimes necessary for certain experimental works. This study presents a technique based on exposure of adult mosquitoes to a blue stroboscopic light for 20 min during several nights, which encourages them to copulate naturally under laboratory conditions. After some generations, a self-free-mating strain was obtained. The technique is simple, inexpensive and is probably effective whatever the An. pseudopunctipennis strain considered.
伪点按蚊是南美洲安第斯地区主要的疟疾传播媒介之一。关于该物种的实验数据很少，因为由于其广适性状态使得在实验室中难以获得，这导致维持种群很困难。实际上，个体在昆虫饲养笼的有限空间内不会交配。为避免这个问题，可以采用强制人工交配。然而，这种技术耗时，需要训练有素的技术人员，并且对于容易进行大规模生产来说并不充分，而大规模生产有时对于某些实验工作是必要的。本研究提出了一种技术，即在几个晚上将成年蚊子暴露于蓝色频闪灯光下20分钟，这促使它们在实验室条件下自然交配。经过几代之后，获得了一个自主交配品系。该技术简单、廉价，并且无论考虑哪种伪点按蚊品系可能都是有效的。
