Villarreal C, Arredondo-Jiménez J I, Rodriguez M H, Ulloa A
Centro de Investigación de Paludismo, Instituto Nacional de Salud Pública, Chiapas, México.
J Am Mosq Control Assoc. 1998 Dec;14(4):369-72.
Two colonies of Anopheles pseudopunctipennis, Tapachula and Abasolo strains, were established under laboratory conditions with a thermoperiod (29 degrees C during the day; 24 degrees C during the night) and artificial dusk. To stimulate mating, a light beam from a flashlight was shone on the cage shortly after lights off. This procedure was repeated for the first 6 mosquito generations (parental to F6) and thereafter light stimulation was unnecessary for mating. The Tapachula colony has been maintained for 24 generations in 24 months, with insemination rates in females > 80% since the F3, and a monthly production of 30,000 pupae since the F7. Using the same procedure, the Abasolo colony from northeastern Mexico has been maintained for 13 generations in 14 months, with insemination rates of 26-52%.
在实验室条件下，利用温度周期（白天29摄氏度；夜间24摄氏度）和人工黄昏建立了两个伪点按蚊群体，即塔帕丘拉株和阿瓦索洛株。为了刺激交配，熄灯后不久用手电筒的光束照射饲养笼。在最初的6代蚊子（亲代至F6）中重复此操作，此后交配无需光照刺激。塔帕丘拉群体在24个月内已维持了24代，自F3代以来雌蚊受精率>80%，自F7代以来每月产蛹30000个。采用相同程序，来自墨西哥东北部的阿瓦索洛群体在14个月内已维持了13代，受精率为26%-52%。
