酵母中端粒酶与短端粒的关联增加。
Increased association of telomerase with short telomeres in yeast.
作者信息
Bianchi Alessandro, Shore David
机构信息
Department of Molecular Biology and National Centre of Competence in Research (NCCR) Frontiers in Genetics Program, University of Geneva, Sciences III, CH-1211 Geneva 4, Switzerland.
出版信息
Genes Dev. 2007 Jul 15;21(14):1726-30. doi: 10.1101/gad.438907.
Abstract
Telomere function is mediated by the assembly of a protein complex on an array of telomeric DNA (TG) repeats synthesized by the telomerase enzyme. Telomerase action at chromosome ends is finely tuned by the telomeric complex so that a constant average number of repeats is maintained. This is achieved through a negative feedback process that is sensitive to TG tract length, but whose underlying mechanism is unknown. We show that short telomeres, which are preferential substrates for telomerase, display increased association with the enzyme in the S phase of the cell cycle, when telomerase acts. In addition, we provide support for a molecular mechanism by which this key step of telomerase recruitment is regulated by TG tract length.
摘要
端粒功能由端粒酶合成的端粒DNA（TG）重复序列阵列上的蛋白质复合体组装介导。端粒复合体精细调节端粒酶在染色体末端的作用，从而维持重复序列的平均数量恒定。这是通过对TG序列长度敏感的负反馈过程实现的，但其潜在机制尚不清楚。我们发现，短端粒是端粒酶的优先底物，在端粒酶发挥作用的细胞周期S期，短端粒与该酶的结合增加。此外，我们为一种分子机制提供了支持，即端粒酶募集的这一关键步骤受TG序列长度调控。
相似文献
1
Increased association of telomerase with short telomeres in yeast.酵母中端粒酶与短端粒的关联增加。
Genes Dev. 2007 Jul 15;21(14):1726-30. doi: 10.1101/gad.438907.
2
Est1p as a cell cycle-regulated activator of telomere-bound telomerase.Est1p作为端粒结合的端粒酶的细胞周期调控激活剂。
Science. 2002 Aug 9;297(5583):1023-6. doi: 10.1126/science.1074968.
3
S. cerevisiae Tel1p and Mre11p are required for normal levels of Est1p and Est2p telomere association.酿酒酵母的端粒酶相关蛋白1（Tel1p）和Mre11蛋白（Mre11p）是Est1p和Est2p正常水平的端粒关联所必需的。
Mol Cell. 2006 Nov 17;24(4):603-10. doi: 10.1016/j.molcel.2006.10.005.
4
Early replication of short telomeres in budding yeast.芽殖酵母中短端粒的早期复制。
Cell. 2007 Mar 23;128(6):1051-62. doi: 10.1016/j.cell.2007.01.041.
5
The yeast Pif1p helicase removes telomerase from telomeric DNA.酵母Pif1p解旋酶从端粒DNA上移除端粒酶。
Nature. 2005 Nov 3;438(7064):57-61. doi: 10.1038/nature04091. Epub 2005 Aug 24.
6
Cdc13 and telomerase bind through different mechanisms at the lagging- and leading-strand telomeres.Cdc13 通过不同的机制与端粒酶结合在滞后链和前导链的端粒上。
Mol Cell. 2010 Jun 25;38(6):842-52. doi: 10.1016/j.molcel.2010.05.016.
7
New function of CDC13 in positive telomere length regulation.CDC13在端粒长度正向调控中的新功能。
Mol Cell Biol. 2001 Jul;21(13):4233-45. doi: 10.1128/MCB.21.13.4233-4245.2001.
8
Regulation of telomere length by an N-terminal region of the yeast telomerase reverse transcriptase.酵母端粒酶逆转录酶N端区域对端粒长度的调控
Mol Cell Biol. 2005 Oct;25(20):9103-14. doi: 10.1128/MCB.25.20.9103-9114.2005.
9
Telomerase repeat addition processivity is increased at critically short telomeres in a Tel1-dependent manner in Saccharomyces cerevisiae.在酿酒酵母中，端粒酶重复序列添加的持续性以依赖于Tel1的方式在极短的端粒处增加。
Genes Dev. 2007 Oct 1;21(19):2485-94. doi: 10.1101/gad.1588807.
10
Tel1 kinase and subtelomere-bound Tbf1 mediate preferential elongation of short telomeres by telomerase in yeast.端粒酶1激酶和与亚端粒结合的Tbf1介导酵母中端粒酶对短端粒的优先延长。
EMBO Rep. 2007 Nov;8(11):1080-5. doi: 10.1038/sj.embor.7401082. Epub 2007 Oct 5.
引用本文的文献
1
Long telomere inheritance through budding yeast sexual cycles.通过芽殖酵母有性周期实现的长端粒遗传。
Genetics. 2025 Sep 3;231(1). doi: 10.1093/genetics/iyaf129.
2
Binding of the TRF2 iDDR motif to RAD50 highlights a convergent evolutionary strategy to inactivate MRN at telomeres.TRF2 iDDR 基序与 RAD50 的结合凸显了一种在端粒处使 MRN 失活的趋同进化策略。
Nucleic Acids Res. 2024 Jul 22;52(13):7704-7719. doi: 10.1093/nar/gkae509.
3
Post-Transcriptional and Post-Translational Modifications in Telomerase Biogenesis and Recruitment to Telomeres.端粒酶生物发生和招募到端粒的转录后和翻译后修饰。
Int J Mol Sci. 2023 Mar 6;24(5):5027. doi: 10.3390/ijms24055027.
4
Break-induced replication: unraveling each step.断裂诱导复制：解析每一步骤
Trends Genet. 2022 Jul;38(7):752-765. doi: 10.1016/j.tig.2022.03.011. Epub 2022 Apr 19.
5
Role and Regulation of Pif1 Family Helicases at the Replication Fork.Pif1家族解旋酶在复制叉处的作用与调控
Int J Mol Sci. 2022 Mar 29;23(7):3736. doi: 10.3390/ijms23073736.
6
Protection of the C. elegans germ cell genome depends on diverse DNA repair pathways during normal proliferation.秀丽隐杆线虫生殖细胞基因组的保护在正常增殖过程中依赖于多种DNA修复途径。
PLoS One. 2021 Apr 27;16(4):e0250291. doi: 10.1371/journal.pone.0250291. eCollection 2021.
7
Tel1/ATM Signaling to the Checkpoint Contributes to Replicative Senescence in the Absence of Telomerase.端粒酶缺失时，Tel1/ATM 信号转导有助于细胞复制性衰老。
Genetics. 2019 Oct;213(2):411-429. doi: 10.1534/genetics.119.302391. Epub 2019 Aug 7.
8
ChECing out Rif1 action in freely cycling cells.在自由循环的细胞中检测Rif1的作用。
Curr Genet. 2019 Apr;65(2):429-434. doi: 10.1007/s00294-018-0902-0. Epub 2018 Nov 19.
9
Effect of therapies-mediated modulation of telomere and/or telomerase on cancer cells radiosensitivity.治疗介导的端粒和/或端粒酶调节对癌细胞放射敏感性的影响。
Oncotarget. 2018 Oct 9;9(79):35008-35025. doi: 10.18632/oncotarget.26150.
10
Fine tuning the level of the Cdc13 telomere-capping protein for maximal chromosome stability performance.精细调整 Cdc13 端粒封端蛋白的水平，以实现最佳的染色体稳定性表现。
Curr Genet. 2019 Feb;65(1):109-118. doi: 10.1007/s00294-018-0871-3. Epub 2018 Jul 31.
本文引用的文献
1
Early replication of short telomeres in budding yeast.芽殖酵母中短端粒的早期复制。
Cell. 2007 Mar 23;128(6):1051-62. doi: 10.1016/j.cell.2007.01.041.
2
DNA breaks are masked by multiple Rap1 binding in yeast: implications for telomere capping and telomerase regulation.酵母中多个Rap1结合掩盖DNA断裂：对端粒封端和端粒酶调控的影响
Genes Dev. 2007 Feb 1;21(3):292-302. doi: 10.1101/gad.400907.
3
S. cerevisiae Tel1p and Mre11p are required for normal levels of Est1p and Est2p telomere association.酿酒酵母的端粒酶相关蛋白1（Tel1p）和Mre11蛋白（Mre11p）是Est1p和Est2p正常水平的端粒关联所必需的。
Mol Cell. 2006 Nov 17;24(4):603-10. doi: 10.1016/j.molcel.2006.10.005.
4
The telomerase-recruitment domain of the telomere binding protein Cdc13 is regulated by Mec1p/Tel1p-dependent phosphorylation.端粒结合蛋白Cdc13的端粒酶招募结构域受Mec1p/Tel1p依赖性磷酸化调控。
Nucleic Acids Res. 2006;34(21):6327-36. doi: 10.1093/nar/gkl786. Epub 2006 Nov 15.
5
The structure and function of telomerase reverse transcriptase.端粒酶逆转录酶的结构与功能。
Annu Rev Biochem. 2006;75:493-517. doi: 10.1146/annurev.biochem.75.103004.142412.
6
The maintenance and masking of chromosome termini.染色体末端的维持与遮蔽
Curr Opin Cell Biol. 2006 Jun;18(3):247-53. doi: 10.1016/j.ceb.2006.04.005. Epub 2006 May 8.
7
Shelterin: the protein complex that shapes and safeguards human telomeres.端粒保护蛋白复合体：塑造并保护人类端粒的蛋白质复合体。
Genes Dev. 2005 Sep 15;19(18):2100-10. doi: 10.1101/gad.1346005.
8
Late S phase-specific recruitment of Mre11 complex triggers hierarchical assembly of telomere replication proteins in Saccharomyces cerevisiae.Mre11复合物在S期晚期的特异性募集触发酿酒酵母端粒复制蛋白的分级组装。
Mol Cell. 2005 Feb 18;17(4):573-83. doi: 10.1016/j.molcel.2005.01.014.
9
SMG7 is a 14-3-3-like adaptor in the nonsense-mediated mRNA decay pathway.SMG7是无义介导的mRNA降解途径中的一种类14-3-3衔接蛋白。
Mol Cell. 2005 Feb 18;17(4):537-47. doi: 10.1016/j.molcel.2005.01.010.
10
Counting of Rif1p and Rif2p on Saccharomyces cerevisiae telomeres regulates telomere length.酿酒酵母端粒上Rif1p和Rif2p的计数调节端粒长度。
Mol Cell Biol. 2004 Dec;24(24):10857-67. doi: 10.1128/MCB.24.24.10857-10867.2004.
Zotero 插件