Malone Emory G, Thompson Matthew D, Byrd Alicia K
Department of Biochemistry and Molecular Biology, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA.
Winthrop P. Rockefeller Cancer Institute, University of Arkansas for Medical Sciences, Little Rock, AR 72205, USA.
Int J Mol Sci. 2022 Mar 29;23(7):3736. doi: 10.3390/ijms23073736.
Pif1 helicases are a multifunctional family of DNA helicases that are important for many aspects of genomic stability in the nucleus and mitochondria. Pif1 helicases are conserved from bacteria to humans. Pif1 helicases play multiple roles at the replication fork, including promoting replication through many barriers such as G-quadruplex DNA, the rDNA replication fork barrier, tRNA genes, and R-loops. Pif1 helicases also regulate telomerase and promote replication termination, Okazaki fragment maturation, and break-induced replication. This review highlights many of the roles and regulations of Pif1 at the replication fork that promote cellular health and viability.
Pif1解旋酶是DNA解旋酶的一个多功能家族，对细胞核和线粒体基因组稳定性的许多方面都很重要。Pif1解旋酶从细菌到人类都是保守的。Pif1解旋酶在复制叉处发挥多种作用，包括促进复制通过许多障碍，如G-四链体DNA、rDNA复制叉障碍、tRNA基因和R环。Pif1解旋酶还调节端粒酶，并促进复制终止、冈崎片段成熟和断裂诱导复制。这篇综述重点介绍了Pif1在复制叉处促进细胞健康和活力的许多作用和调控机制。
